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Sample GSM3136749 Query DataSets for GSM3136749
Status Public on Jun 20, 2018
Title MBC32
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: medullary breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.5670856040816
1053_at 7.84453057721313
117_at 7.61772803509937
121_at 7.62238414461416
1255_g_at 3.13826817618919
1294_at 8.08240730801673
1316_at 4.891607768007
1320_at 4.23651645603497
1405_i_at 10.8773789304069
1431_at 3.73630693941964
1438_at 7.36784066615551
1487_at 7.62158605703276
1494_f_at 5.23233475903782
1552256_a_at 9.76299306190324
1552257_a_at 7.64864691500244
1552258_at 4.81897666062579
1552261_at 4.70241983068243
1552263_at 5.57502493904446
1552264_a_at 7.01991018348197
1552266_at 4.09895367284223

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136749_TM01_ARN0011_s1h1s1_U133p2.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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