|
| Status |
Public on Jun 04, 2020 |
| Title |
wox5_2: wox5-1 rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
Arabidopsis root tissue
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: root
genotype/variation: wox5-1
age: 5 days
|
| Growth protocol |
Seeds were vernalized and imbibed at 4C for 2 days, then plated on 1xMS, 1% sucrose and grown vertically in 16 hr light/8 hr dark for 5 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For AGL42:GFP roots, GFP positive and negative cells were collected in a solution of B-mercaptoethanol and RLT buffer using a MoFlo sorter. For the rest, approximately 3mm of root tissue was collected in a solution of of B-mercaptoethanol and RLT buffer
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Reads filtered using ea-utils
Reads aligned using Bowtie and Cufflinks
FPKM values obtained using Cufflinks
Genome_build: Tair10
Supplementary_files_format_and_content: FPKM values for each biological replicate
|
| |
|
| Submission date |
May 04, 2018 |
| Last update date |
Jun 04, 2020 |
| Contact name |
Natalie M Clark |
| E-mail(s) |
nmclark2@iastate.edu, nclark@broadinstitute.org
|
| Organization name |
Iowa State University
|
| Department |
Plant Pathology and Microbiology
|
| Lab |
Walley Lab
|
| Street address |
Advanced Teaching and Research Building 2213 Pammel Drive
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platform ID |
GPL17639 |
| Series (1) |
| GSE104945 |
Transcriptional profile of wox5-1 and seu-3 mutants |
|
| Relations |
| BioSample |
SAMN09069681 |
| SRA |
SRX4040835 |
