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Sample GSM3108692 Query DataSets for GSM3108692
Status Public on Jun 29, 2018
Title human iPSC which is used to induce cancer stem cells
Sample type RNA
Source name original human iPSC 201B7
Organism Homo sapiens
Characteristics gender: Female
age: 36
Treatment protocol Human iPS cells obtained from Riken.
Extracted molecule total RNA
Extraction protocol Using the RNeasy column purification (QIAGEN) total RNA was prepared.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 100 ng RNA using Low Input Quick Amp Labeling Kit, One-Color (Agilent Technologies) according to the manufacturer's instructions.
Hybridization protocol 600 ng of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes and hybridized for 17 hours at 65°C with Gene Expression Hybridization Kit (Agilent Technologies).
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2565CA) using one color scan setting for 8x60K array slides.
Description Gene expression of induced cancer stem cell induced from human iPS cell by ZR-75-1 cell culture medium
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent Technologies) using parameters (protocol GE1_1010_Sep10 and Grid: 039494_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Submission date Apr 23, 2018
Last update date Jul 01, 2018
Contact name Akimasa Seno
Organization name Okayama University
Department Applied Chemistry and Biotechnology
Lab Nano-Biotechnology
Street address 1-1, Tsushima-Naka, 1-chome, Kita-Ku
City Okayama
State/province Okayama
ZIP/Postal code 7008530
Country Japan
Platform ID GPL17077
Series (1)
GSE113533 Gene expression analysis of cancer stem cells with stem cells or cancer related cells

Data table header descriptions
VALUE Normalized signal intensity by Agilp

Data table
A_23_P117082 10.43435946
A_33_P3246448 5.951661716
A_33_P3318220 5.591901845
A_33_P3236322 5.423620572
A_33_P3319925 5.728652515
A_21_P0000509 17.40654344
A_21_P0000744 8.387536641
A_24_P215804 6.680691255
A_23_P110167 9.39782818
A_33_P3211513 7.236421061
A_23_P103349 5.553702903
A_32_P61480 5.498051554
A_33_P3788124 7.063197841
A_33_P3414202 6.586328622
A_33_P3316686 7.433371769
A_33_P3300975 5.649740675
A_33_P3263061 11.46208947
A_33_P3261373 5.750860118
A_24_P278460 8.87987974
A_21_P0013109 5.412594181

Total number of rows: 50170

Table truncated, full table size 1245 Kbytes.

Supplementary file Size Download File type/resource
GSM3108692_hiPSC.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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