|
| Status |
Public on Dec 31, 2008 |
| Title |
HepG2 cells stimulated with AFP |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Control HepG2 cells, untreated
|
| Organism |
Homo sapiens |
| Characteristics |
HepG2 cells were cultured without 5,000 ng/ml of human cord blood AFP for 24 hours.
|
| Treatment protocol |
Five million HepG2 cells were cultured with or without 5,000 ng/ml of human cord blood AFP for 24 hours. Five million HLE cells were cultured with or without 5,000 ng/ml of human cord blood AFP for 24 hours.
|
| Growth protocol |
HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium containg 10% fetal bovine serum. HLE cells were cultured in Dulbecco's Modified Eagle's Medium containg 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Rneasy (Qiagen) following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol.
|
| |
|
| Channel 2 |
| Source name |
HepG2 cells stimulated with AFP
|
| Organism |
Homo sapiens |
| Characteristics |
HepG2 cells were cultured with 5,000 ng/ml of human cord blood AFP for 24 hours.
|
| Treatment protocol |
Five million HepG2 cells were cultured with or without 5,000 ng/ml of human cord blood AFP for 24 hours. Five million HLE cells were cultured with or without 5,000 ng/ml of human cord blood AFP for 24 hours.
|
| Growth protocol |
HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium containg 10% fetal bovine serum. HLE cells were cultured in Dulbecco's Modified Eagle's Medium containg 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Rneasy (Qiagen) following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol.
|
| |
|
| |
| Hybridization protocol |
Agilent two-color gene expression hybridization/wash protocol.
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 9.5.3
|
| Description |
HepG2 cell line
|
| Data processing |
LOWESS normalized, background-subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Jul 31, 2008 |
| Last update date |
Aug 05, 2008 |
| Contact name |
Noboru Mitsuhashi |
| E-mail(s) |
nmitsuhashi@faculty.chiba-u.jp
|
| Phone |
+81-43-290-3124
|
| Organization name |
Research Center for Frontier Medical Engineering, Chiba University
|
| Department |
Section for Medical Nanotechniques
|
| Street address |
1-33 Yayoi-cho, Inage-ku
|
| City |
Chiba |
| ZIP/Postal code |
263-8522 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE12307 |
siRNA Administration Against AFP Inhibits Hepatocellular Carcinoma Proliferation in Vitro and Progression in Mice |
|
