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Sample GSM3084603 Query DataSets for GSM3084603
Status Public on May 01, 2019
Title RNA_1C_Setd2_KO_RNA_rep2
Sample type SRA
 
Source name embryo
Organism Mus musculus
Characteristics tissue: embryo
developmental stage: 1C
genotype: Setd2_KO
Treatment protocol 8-week-old females were intraperitoneally injected with PMSG and hCG. Oocytes and embryos were harvested in M2 medium at following time point after hCG: MII oocytes, 13h; Zygotes 30 h; late-two cell embryos, 47 h; blastocyst, 98 h. Germinal versicle stage (GV) oocytes were harvested at 48 h after PMSG. For D7, D10, D14 oocytes, corresponding postnatal female mouse were sacrificed and oocytes were harvested in M2.
Extracted molecule total RNA
Extraction protocol The RNA-seq libraries were generated following the Smart-seq2 protocol with minor modification (Picelli et al., 2014).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Description processed data file:
Setd2_KO_RNA_FPKM.txt.gz
Data processing RNA-seq datasets were mapped to the mouse mm9 reference genome by Tophat 2.0.11. Gene expression was then calculated according to refFlat database by cufflinks 2.0.2.
Genome_build: mm9
Supplementary_files_format_and_content: The RNA FPKM txt file contains FPKM value for each sample.
 
Submission date Apr 06, 2018
Last update date May 01, 2019
Contact name Wei Xie
E-mail(s) xiewei121@tsinghua.edu.cn
Organization name Tsinghua University
Department School of Life Science
Street address Haidian District
City Beijing
ZIP/Postal code 100084
Country China
 
Platform ID GPL21273
Series (2)
GSE112832 SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development [RNA-seq]
GSE112835 SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development
Relations
BioSample SAMN08887565
SRA SRX3899469

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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