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Sample GSM3080184 Query DataSets for GSM3080184
Status Public on Apr 04, 2019
Title TOFU-5 ChIP-seq
Sample type SRA
 
Source name Young adults
Organism Caenorhabditis elegans
Characteristics tissue: whole animal extract
developmental stage: Young adults
Growth protocol 20 C, in NGM plates.
Extracted molecule genomic DNA
Extraction protocol Animals were frozen in liquid nitogen and crushed in metallic grinder. Animal extracts were lysed in FA Buffer and sonicated in BIORUPTOR at 4C.
NEB-NEXT Ultra II DNA Prep
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 1500
 
Data processing read trimming
mapping to genome with BWA
removal of blacklisted areas
MACS2 peak calling with a summed input, p value cutoff 0.01
preparation of normalized signal tracks with BEADs algorithm.
Genome_build: ce10
Supplementary_files_format_and_content: bw, narrowpeak
 
Submission date Apr 04, 2018
Last update date Apr 04, 2019
Contact name Eric Miska
E-mail(s) eam29@cam.ac.uk
Organization name University of Cambridge
Department The Gurdon Institute
Lab Eric Miska Lab
Street address tennis court road
City cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
 
Platform ID GPL18730
Series (1)
GSE112682 Functional proteomics identified an upstream trancription complex promoting piRNA biogenesis
Relations
BioSample SAMN08863774
SRA SRX3883438

Supplementary file Size Download File type/resource
GSM3080184_TOFU5_processed_data.tar.gz 512.5 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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