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Status |
Public on Apr 04, 2019 |
Title |
TOFU-5 ChIP-seq |
Sample type |
SRA |
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Source name |
Young adults
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Organism |
Caenorhabditis elegans |
Characteristics |
tissue: whole animal extract developmental stage: Young adults
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Growth protocol |
20 C, in NGM plates.
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Extracted molecule |
genomic DNA |
Extraction protocol |
Animals were frozen in liquid nitogen and crushed in metallic grinder. Animal extracts were lysed in FA Buffer and sonicated in BIORUPTOR at 4C. NEB-NEXT Ultra II DNA Prep
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Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 1500 |
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Data processing |
read trimming mapping to genome with BWA removal of blacklisted areas MACS2 peak calling with a summed input, p value cutoff 0.01 preparation of normalized signal tracks with BEADs algorithm. Genome_build: ce10 Supplementary_files_format_and_content: bw, narrowpeak
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Submission date |
Apr 04, 2018 |
Last update date |
Apr 04, 2019 |
Contact name |
Eric Miska |
E-mail(s) |
eam29@cam.ac.uk
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Organization name |
University of Cambridge
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Department |
The Gurdon Institute
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Lab |
Eric Miska Lab
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Street address |
tennis court road
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City |
cambridge |
ZIP/Postal code |
CB2 1QN |
Country |
United Kingdom |
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Platform ID |
GPL18730 |
Series (1) |
GSE112682 |
Functional proteomics identified an upstream trancription complex promoting piRNA biogenesis |
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Relations |
BioSample |
SAMN08863774 |
SRA |
SRX3883438 |
Supplementary file |
Size |
Download |
File type/resource |
GSM3080184_TOFU5_processed_data.tar.gz |
512.5 Mb |
(ftp)(http) |
TAR |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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