|
| Status |
Public on Oct 10, 2018 |
| Title |
SA01 |
| Sample type |
SRA |
| |
|
| Source name |
Hippocampus_saline
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: post natal day 14
treated with: Saline (i.p. injection) for 2 hours
tissue: Hippocampus
|
| Treatment protocol |
Mice were treated with saline or 10 mg/kg LPS for two hours.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Single cells were captured and lysed on the 10x Chromium Controller instrument (10x Genomics).
Standard 10x Genomics scRNA-seq library construction protocol was used to construct the library
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The sequencing data were was processed with the 10X Cell Ranger v2.1.
Genome_build: mm10
Supplementary_files_format_and_content: csv files contain per-gene, per-cell expression and were created using cellranger mat2csv
|
| |
|
| Submission date |
Mar 28, 2018 |
| Last update date |
Oct 12, 2018 |
| Contact name |
Xiang Yu |
| E-mail(s) |
yuxiang@ion.ac.cn
|
| Organization name |
Institute of Neuroscience, Chinese Academy of Sciences
|
| Street address |
320 Yue Yang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE112436 |
PDGFRβ Cells Rapidly Relay Inflammatory Signal from the Circulatory System to Neurons via Chemokine CCL2 |
|
| Relations |
| BioSample |
SAMN08804973 |
| SRA |
SRX3856556 |
