|
Status |
Public on Jun 04, 2009 |
Title |
Renal Cell Carcinoma 061507B |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
mRNA from Renal Clear Cell Carcinoma
|
Organism |
Homo sapiens |
Characteristics |
Organism Part: Kidney BioSourceType: Frozen Sample BioSource Provider: University of North Carolina
|
Growth protocol |
Frozen RCCs were obtained through University of North Carolina - Chapel Hill's Tissue Procurement Facility
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracting with Qiagen RNeasy kit.
|
Label |
Cy5
|
Label protocol |
Tumor sample RNA and reference RNA was labelled by UNC Lineberger Comprehensive Cancer Center Genomics Core
|
|
|
Channel 2 |
Source name |
Perou lab reference RNA
|
Organism |
Homo sapiens |
Characteristics |
BioSourceType: Cancer cell lines
|
Growth protocol |
Frozen RCCs were obtained through University of North Carolina - Chapel Hill's Tissue Procurement Facility
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracting with Qiagen RNeasy kit.
|
Label |
Cy3
|
Label protocol |
Tumor sample RNA and reference RNA was labelled by UNC Lineberger Comprehensive Cancer Center Genomics Core
|
|
|
|
Hybridization protocol |
Tumor sample RNA and reference RNA were hybridized on Agilent Whole Human Genome 4x44k arrays by UNC Lineberger Comprehensive Cancer Center Genomics Core
|
Scan protocol |
Agilent gene chips were scanned on an Agilent scanner by UNC Lineberger Comprehensive Cancer Center Genomics Core
|
Description |
Gene expression data from Human RCC sample RNA quality was determined using an Agilent LabChip Bioanalyzer. H&E was performed on tumor tissue samples to confirm clear cell histopathology
|
Data processing |
LOWESS normalized, background subtracted VALUE data obtained from log2 of processed Red signal/processed Green signal. UNC Microarry Database software was used.
|
|
|
Submission date |
Jul 15, 2008 |
Last update date |
Jun 04, 2009 |
Contact name |
Kimryn Rathmell |
E-mail(s) |
kimryn.rathmell@vanderbilt.edu
|
Organization name |
Vanderbilt
|
Department |
Medicine
|
Lab |
Rathmell
|
Street address |
2220 Pierce Ave
|
City |
Nashville |
State/province |
TN |
ZIP/Postal code |
37232 |
Country |
USA |
|
|
Platform ID |
GPL4133 |
Series (1) |
GSE12114 |
Ror2 gene expression analysis in renal cell carcinoma |
|