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Sample GSM305517 Query DataSets for GSM305517
Status Public on Jun 04, 2009
Title Renal Cell Carcinoma 067116B
Sample type RNA
 
Channel 1
Source name mRNA from Renal Clear Cell Carcinoma
Organism Homo sapiens
Characteristics Organism Part: Kidney
BioSourceType: Frozen Sample
BioSource Provider: University of North Carolina
Growth protocol Frozen RCCs were obtained through University of North Carolina - Chapel Hill's Tissue Procurement Facility
Extracted molecule total RNA
Extraction protocol RNA was extracting with Qiagen RNeasy kit.
Label Cy5
Label protocol Tumor sample RNA and reference RNA was labelled by UNC Lineberger Comprehensive Cancer Center Genomics Core
 
Channel 2
Source name Perou lab reference RNA
Organism Homo sapiens
Characteristics BioSourceType: Cancer cell lines
Growth protocol Frozen RCCs were obtained through University of North Carolina - Chapel Hill's Tissue Procurement Facility
Extracted molecule total RNA
Extraction protocol RNA was extracting with Qiagen RNeasy kit.
Label Cy3
Label protocol Tumor sample RNA and reference RNA was labelled by UNC Lineberger Comprehensive Cancer Center Genomics Core
 
 
Hybridization protocol Tumor sample RNA and reference RNA were hybridized on Agilent Whole Human Genome 4x44k arrays by UNC Lineberger Comprehensive Cancer Center Genomics Core
Scan protocol Agilent gene chips were scanned on an Agilent scanner by UNC Lineberger Comprehensive Cancer Center Genomics Core
Description Gene expression data from Human RCC sample
RNA quality was determined using an Agilent LabChip Bioanalyzer. H&E was performed on tumor tissue samples to confirm clear cell histopathology
Data processing LOWESS normalized, background subtracted VALUE data obtained from log2 of processed Red signal/processed Green signal. UNC Microarry Database software was used.
 
Submission date Jul 15, 2008
Last update date Jun 04, 2009
Contact name Kimryn Rathmell
E-mail(s) kimryn.rathmell@vanderbilt.edu
Organization name Vanderbilt
Department Medicine
Lab Rathmell
Street address 2220 Pierce Ave
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
 
Platform ID GPL4133
Series (1)
GSE12114 Ror2 gene expression analysis in renal cell carcinoma

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
1 0.329
2 -2
3 -1.737
4 -2
5 -1.586
6 -1.737
7 0
8 -1.377
9 -1.171
10 -1.171
11 -0.415
12 -1.766
13 -0.808
14 0.227
15 -0.744
16 -0.496
17 0.451
18 -0.085
19 0.972
20 0

Total number of rows: 45220

Table truncated, full table size 458 Kbytes.




Supplementary file Size Download File type/resource
GSM305517.txt.gz 6.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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