|
Status |
Public on Mar 13, 2018 |
Title |
MDA-MB-231_ATCC_1 |
Sample type |
genomic |
|
|
Source name |
cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: MDA-MB-231_ATCC cell type: breast cancer cell line gfp: no fluorescent proteins
|
Treatment protocol |
Untreated cells
|
Growth protocol |
Cells grown in DMEM 10% FBS, 1% P/S
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was made using Qiagen DNA mini kit and concentration determined using a Nanodrop Spectrophotometer
|
Label |
Cy5/Cy3
|
Label protocol |
Bisulfite conversion of genomic DNA samples was performed using the Zymo EZ DNA Methylation kit. 200ng of bisulfite-converted DNA was then prepared for hybridisation according to the Illumina Infinium HD Assay Methylation Protocol Guide.
|
|
|
Hybridization protocol |
Samples were hybridised to Illumina MethylationEPIC arrays for 17 hours at 48°C in an Illumina Hybridisation Oven. Single base extension and staining were performed as per the Illumina Infinium HD Protocol.
|
Scan protocol |
Slides were scanned using an Illumina HiScan.
|
Description |
SAMPLE 1
|
Data processing |
Genomic DNA extracted from cell lines was genotyped using Infinium HumanCytoSNP-12 v2.1 BeadChip arrays (Illumina)
|
|
|
Submission date |
Mar 12, 2018 |
Last update date |
Mar 13, 2018 |
Contact name |
Andrew Pattison |
E-mail(s) |
andrew.pattison@monash.edu
|
Organization name |
Monash University
|
Street address |
Wellington Rd
|
City |
Melbourne |
State/province |
Vic |
ZIP/Postal code |
3800 |
Country |
Australia |
|
|
Platform ID |
GPL13829 |
Series (2) |
GSE111705 |
Functional and genomic characterization of a xenograft model system for the study of metastasis in triple-negative breast cancer |
GSE111706 |
Metastasis in triple-negative breast cancer |
|