|
Status |
Public on Nov 30, 2008 |
Title |
Ad-F7 transduction + IgG treatment in primary human macrophages (Experiment 2) |
Sample type |
RNA |
|
|
Source name |
Macrophages Ad-F7 + IgG
|
Organism |
Homo sapiens |
Characteristics |
Human Macrophages transduced with adenoviral vector expression the active form of IRF-7 (IRF-7 D247-467; Ad-F7) and treatment of cells with control isotype (IgG). RNA collected at 24 hours.
|
Treatment protocol |
Transduction of primary human macrophages with Ad-F7 and treatment of cells with IgG
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy Plus extraction kit
|
Label |
Cy3
|
Label protocol |
Ambion, TotalPrep, for biotin labeling and illumina's protocol for Cy3 labeling.
|
|
|
Hybridization protocol |
From illumina
|
Scan protocol |
From illumina
|
Description |
See illumina's sample preparation
|
Data processing |
BeadStudio from illumina, J-Express from Molmine and R
|
|
|
Submission date |
Jul 03, 2008 |
Last update date |
Sep 24, 2008 |
Contact name |
Eduardo Hernandez |
E-mail(s) |
camacho99@yahoo.com
|
Organization name |
Lady Davis Institute for Medical Research (JGH)
|
Department |
Molecular Oncology Group
|
Lab |
526
|
Street address |
3755 Cote-Sainte-Catherine Road
|
City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3T-1E2 |
Country |
Canada |
|
|
Platform ID |
GPL6883 |
Series (2) |
GSE12002 |
Transcriptional profiles of Ad-F7 transduced macrophages treated with anti-IFNAR2 antibody or control isotype (IgG) |
GSE12120 |
Transcriptional re-programming of primary human macrophages by IRF-3 and IRF-7 |
|