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Sample GSM302946 Query DataSets for GSM302946
Status Public on Jul 30, 2008
Title HeLa cells transfected with miR-1 duplex versus control transfected HeLa, 24 hours
Sample type RNA
 
Channel 1
Source name Mock transfected HeLa cells, 24 hours
Organism Homo sapiens
Characteristics Expression profile of mRNAs from mock-transfected HeLa cells for 24 hours
Treatment protocol Cells grown in SILAC media containing heavy amino acids (Arg and Lys) were mock-transfected with lipofectamine 2000 (Invitrogen).
Growth protocol HeLa cells were grown in SILAC DMEM media.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy Plus
Label Cy3
Label protocol Agilent 1 Cycle Labelling Standard Protocol
 
Channel 2
Source name HeLa cells transfected with miR-1 duplex, 24 hours
Organism Homo sapiens
Characteristics Expression profile of mRNAs from HeLa cells transfected with miR-1 duplex for 24 hours
Treatment protocol Cells grown in SILAC media containing light amino acids (Arg and Lys) were transfected with miRNA-1 duplexes.
Growth protocol HeLa cells were grown in SILAC DMEM media.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy Plus
Label Cy5
Label protocol Agilent 1 Cycle Labelling Standard Protocol
 
 
Hybridization protocol Agilent Sure Hybe Protocol
Scan protocol Agilent Scanner
Description Expression profiling comparison of mRNAs in miRNA-1-transfected HeLa cells vs. mock-transfected HeLa cells
Data processing Agilent Feature Extraction Software, Protocol: GE2-v5_95_Feb07
 
Submission date Jul 02, 2008
Last update date Jul 15, 2008
Contact name Whitehead Institute
E-mail(s) sgupta@wi.mit.edu
Phone 617-324-0339
Organization name Whitehead Institute
Department Genome Technology Core
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
 
Platform ID GPL4133
Series (2)
GSE11968 Analysis of HeLa cells after transfection with miR-1, miR-124 and miR-181.
GSE12075 The impact of microRNAs on protein output

Data table header descriptions
ID_REF
VALUE Fold Change (Log2) = Log2( Cy5 / Cy3 )
Fold Change Error Fold Change Error (Log2)
P Value P Value
Cy5 Signal Intensity Cy5 Signal Intensity (miR-1 Transfected)
Cy3 Signal Intensity Cy3 Signal Intensity (Mock Transfected)

Data table
ID_REF VALUE Fold Change Error P Value Cy5 Signal Intensity Cy3 Signal Intensity
1 0.541080 0.209930 0.009954 68351.450000 46974.950000
2 0.000000 2.063574 1.000000 3.328142 4.398629
3 0.000000 2.066162 1.000000 3.295680 4.403583
4 0.000000 2.068471 1.000000 3.269283 4.409678
5 0.000000 2.070582 1.000000 3.247446 4.417030
6 0.000000 2.072379 1.000000 3.230837 4.425113
7 0.000000 2.073937 1.000000 3.218282 4.434063
8 0.000000 2.075239 1.000000 3.209619 4.443677
9 0.000000 2.076287 1.000000 3.204472 4.453716
10 0.000000 2.077124 1.000000 3.202368 4.464399
11 0.000000 2.077739 1.000000 3.203107 4.475395
12 -0.085733 0.204846 0.675563 454.673400 482.511700
13 -1.100266 0.245278 0.000007 56.687140 121.534000
14 -0.296318 0.206067 0.150443 685.740500 842.093900
15 -0.603516 0.327749 0.065563 25.304660 38.448270
16 0.563386 0.210420 0.007419 64388.820000 43572.680000
17 -0.161180 0.205778 0.433469 331.547800 370.737100
18 -0.111747 0.206967 0.589246 227.959200 246.318200
19 1.000241 0.223616 0.000008 108440.100000 54210.990000
20 -0.110876 0.217219 0.609747 103.325900 111.579900

Total number of rows: 45015

Table truncated, full table size 2435 Kbytes.




Supplementary file Size Download File type/resource
GSM302946.txt.gz 15.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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