|
Status |
Public on Oct 15, 2008 |
Title |
Peripheral T cell lymphoma, unspecified patient (PTCL u8) |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Human Universal Reference Total RNA (Beckton Dickinson)
|
Organism |
Homo sapiens |
Characteristics |
Human Universal Reference Total RNA
|
Biomaterial provider |
Beckton Dickinson
|
Extracted molecule |
total RNA |
Extraction protocol |
N/A
|
Label |
Cy3
|
Label protocol |
Cy3-labeled cRNA was generated from 0.5 ug of total RNA according to the manufacturer's protocol (Agilent Technology).
|
|
|
Channel 2 |
Source name |
Total RNA from frozen stomach tissue
|
Organism |
Homo sapiens |
Characteristics |
Stomach from peripheral T-cell lymphoma, unspecified patient (PTCL u8)
|
Extracted molecule |
total RNA |
Extraction protocol |
Frozen lymphoma tissues were ground to powder and lysed in lysis buffer Trizol reagent (Invitrogen).
|
Label |
Cy5
|
Label protocol |
Cy5-labeled cRNA was generated from 0.5 ug of total RNA according to the manufacturer's protocol (Agilent Technology).
|
|
|
|
Hybridization protocol |
The probe consisted of a mixture of Cy5-labeled patient's cRNA and Cy3-labeled control cRNA was hybridized for 17 hours at 65C on the Agilent 44K human oligo microarray.
|
Scan protocol |
Hybridized array slides were scanned at 10-um resolution using an Agilent G2565BA fluorescence dual laser scanner for Cy3 and Cy5 excitation.
|
Description |
Gene expression data from lymphoma tissue of a peripheral T-cell lymphoma, unspecified patient
|
Data processing |
Image intensity data were recorded and transferred into text files using Feature Extraction software, version 9.1.
|
|
|
Submission date |
Jul 01, 2008 |
Last update date |
Oct 15, 2008 |
Contact name |
Kana Miyazaki |
E-mail(s) |
k-myzk@clin.medic.mie-u.ac.jp
|
Phone |
81-59-231-5418
|
Organization name |
Mie University
|
Department |
Department of Hematology and Oncology
|
Street address |
2-174 Edobashi
|
City |
Tsu |
State/province |
Mie |
ZIP/Postal code |
514-8507 |
Country |
Japan |
|
|
Platform ID |
GPL4133 |
Series (1) |
GSE11946 |
Gene expression profiling of peripheral T-cell lymphoma including gamma delta T-cell lymphoma |
|