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Sample GSM3021110 Query DataSets for GSM3021110
Status Public on Jun 30, 2019
Title Involved epidermis from AD skin, sample 5, set 2
Sample type RNA
Source name Human involved epidermis from AD skin, sample 5 (laser capture microdissection)
Organism Homo sapiens
Characteristics tissue: epidermis
disease: atopic dermatitis
Treatment protocol Epidermis and dermis were obtained from human skin biopsies by laser capture microdissection using Arcturus XT (MDS Analytical Technologies) system
Extracted molecule total RNA
Extraction protocol RNA was isolated with Arcturus Picopure RNA isolation Kit according to manufacturer's protocol
Label Cy3
Label protocol 10 ng RNA were amplified with ExpressArt Kit and labeled (Cy3) with One-color microarray-based Gene Expression Analysis Low Input Quick Amp WT Labeling (Agilent Technologies) according to manufacturer's protocol
Hybridization protocol 1.5 ug of Cy3-labelled cRNA was hybridized with Human Gene Expression Microarray 8x60K chip (Agilent Technologies) according to manufacturer's protocol
Scan protocol Slides were scanned with the Agilent G2505B scanner
Description Gene expression in human epidermis
Data processing Images were quantified using Agilent Feature Extraction Software (version V. Data were background-corrected with 'normexp' option and quantile normalized using limma R package
Submission date Feb 23, 2018
Last update date Jul 01, 2019
Contact name Denis A Mogilenko
Organization name INSERM
Department U1011
Street address 1 Rue du Professeur Calmette, BP245
City Lille
ZIP/Postal code 59019
Country France
Platform ID GPL13607
Series (2)
GSE111054 Gene expression in uninvolved and involved epidermis from patients with atopic dermatitis (laser capture microdissection): set 2
GSE111055 Gene expression in uninvolved and involved lesional epidermis from human skin biopsies from patients with atopic dermatitis (laser capture microdissection)

Data table header descriptions
VALUE Normalized log2 expression levels

Data table
1 15.60531399
2 5.186061258
3 5.001347976
4 5.839957397
5 5.87110223
6 6.180504214
7 9.452560252
8 10.25284861
9 5.186061258
10 6.238976221
11 5.345357803
12 7.93828528
13 8.360429742
14 10.93149833
15 8.255759947
16 5.309427714
17 6.823748253
18 5.574553053
19 5.309427714
20 7.712732733

Total number of rows: 62976

Table truncated, full table size 1091 Kbytes.

Supplementary file Size Download File type/resource
GSM3021110_DN5EL.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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