|
| Status |
Public on Jun 30, 2019 |
| Title |
Involved epidermis from AD skin, sample 3, set 1 |
| Sample type |
RNA |
| |
|
| Source name |
Human involved epidermis from AD skin, sample 3 (laser capture microdissection)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: epidermis
disease: atopic dermatitis
|
| Treatment protocol |
Epidermis and dermis were obtained from human skin biopsies by laser capture microdissection using Arcturus XT (MDS Analytical Technologies) system
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Arcturus Picopure RNA isolation Kit according to manufacturer's protocol
|
| Label |
Cy3
|
| Label protocol |
10 ng RNA were amplified with ExpressArt Kit and labeled (Cy3) with One-color microarray-based Gene Expression Analysis Low Input Quick Amp WT Labeling (Agilent Technologies) according to manufacturer's protocol
|
| |
|
| Hybridization protocol |
1.5 ug of Cy3-labelled cRNA was hybridized with Human Gene Expression Microarray 8x60K chip (Agilent Technologies) according to manufacturer's protocol
|
| Scan protocol |
Slides were scanned with the Agilent G2505B scanner
|
| Description |
Gene expression in human epidermis
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version V.10.7.3.1). Data were background-corrected with 'normexp' option and quantile normalized using limma R package
|
| |
|
| Submission date |
Feb 23, 2018 |
| Last update date |
Jul 01, 2019 |
| Contact name |
Denis A Mogilenko |
| Organization name |
INSERM
|
| Department |
U1011
|
| Street address |
1 Rue du Professeur Calmette, BP245
|
| City |
Lille |
| ZIP/Postal code |
59019 |
| Country |
France |
| |
|
| Platform ID |
GPL13607 |
| Series (2) |
| GSE111053 |
Gene expression in uninvolved and involved epidermis from patients with atopic dermatitis (laser capture microdissection): set 1 |
| GSE111055 |
Gene expression in uninvolved and involved lesional epidermis from human skin biopsies from patients with atopic dermatitis (laser capture microdissection) |
|
