|
Status |
Public on Aug 08, 2019 |
Title |
8h_rep1 (Pulse-Chase) |
Sample type |
RNA |
|
|
Source name |
mouse ESCs
|
Organism |
Mus musculus |
Characteristics |
cell type: ESCs treatment: 8h treatment with 4OHT
|
Treatment protocol |
cells were stimulated with 250nM 4OHT, or treated and subsequently inhibited with 1μM PD0325901
|
Growth protocol |
Cells were grown in standard ESC medium supplemented with LIF and 250nM PD173074
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was collected using the RNeasy Mini Kit (Qiagen). Genomic DNA was eliminated by DNAse treatment (Qiagen).
|
Label |
Cy5
|
Label protocol |
RNA was amplified into cRNA and 100ng of total RNA was labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies- 5190-2305).
|
|
|
Hybridization protocol |
Samples were then hybridized to the Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (60000 probes, Agilent Technologies).
|
Scan protocol |
Images were scanned using the high sensitivity protocol (AgilentG3_HiSen_GX_1color) on a SureScan microarray scanner (Agilent Technologies) and probe intensities were obtained by taking the gProcessedSignal from the output of Agilent feature extraction software using default settings.
|
Description |
Gene expression data from 8h treated cells
|
Data processing |
Probe intensities were analyzed using the NIA Array Analysis tool (http://lgsun.grc.nia.nih.gov/ANOVA/).
|
|
|
Submission date |
Feb 15, 2018 |
Last update date |
Aug 08, 2019 |
Contact name |
Josh M Brickman |
E-mail(s) |
joshua.brickman@sund.ku.dk
|
Organization name |
Copenhagen University
|
Department |
reNEW
|
Lab |
Brickman
|
Street address |
3B Blegdamsvej
|
City |
Copenhagen |
ZIP/Postal code |
2200 |
Country |
Denmark |
|
|
Platform ID |
GPL13912 |
Series (2) |
GSE110662 |
Erk-dependent transcriptome |
GSE132444 |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation |
|