|
Status |
Public on Jun 04, 2020 |
Title |
Infected-hLEC-2 |
Sample type |
SRA |
|
|
Source name |
primary human lymphatic endothelial cell
|
Organism |
Homo sapiens |
Characteristics |
cell type: primary human lymphatic endothelial cell treatment: Mycobacterium tuberculosis infected
|
Treatment protocol |
Mtb cultures were grown to exponential phase before being used to infect hLECs at MOI of 10 for 5 hours. Cells were washed free of Mtb with PBS and cultured for 48h with gentamicin.
|
Growth protocol |
Primary hLEC were cultured up to 5 passages in complete endothelial cell medium
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were lysed with TRIzol and total RNA was extracted using Direct-zol RNA miniprep kit RNA-Seq libraries were constructed using TruSeq Stranded Total RNA Library Prep Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Trimmomatic v0.36 STAR v2.5.2a RSEM v1.2.29 DESeq2 v1.18.1 Genome_build: GRCh38 release 88
|
|
|
Submission date |
Feb 13, 2018 |
Last update date |
Mar 29, 2023 |
Contact name |
Rachel Lai |
E-mail(s) |
rachel.lai@imperial.ac.uk
|
Organization name |
Imperial College London
|
Department |
Infectious Disease
|
Street address |
Du Cane Road
|
City |
London |
ZIP/Postal code |
W12 0NN |
Country |
United Kingdom |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE110564 |
Mycobacterium tuberculosis cording in lymphatic endothelial cells (hLEC) |
|
Relations |
BioSample |
SAMN08529092 |
SRA |
SRX3691939 |