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Sample GSM2991666 Query DataSets for GSM2991666
Status Public on Nov 22, 2019
Title FetalTreg4
Sample type SRA
 
Source name Fetal spleenocytes
Organism Homo sapiens
Characteristics cell type: fetal Treg cells
tissue: Fetal spleenocytes
age: 23.0 weeks
Sex: Male
Extracted molecule total RNA
Extraction protocol Cells were sorted by FACS, and total RNA was extracted from 250,000 cells using the Nucleospin RNA kit (Machery Nagel)
Libraries were constructed using a standard Illumina protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Description FT4
Data processing Fastq files were mapped to the genome using Bowtie 2
Read counts per gene was obtained using RSEM
TMM normalization and differential expression analysis performed using EdgeR package for Bioconductor
Genome_build: hg19
Supplementary_files_format_and_content: TMM-normalized data.
 
Submission date Feb 12, 2018
Last update date Nov 24, 2019
Contact name Melissa Ng
E-mail(s) melissa.ng@ucsf.edu
Organization name University of California, San Francisco
Lab Burt Lab
Street address 35 Medical Centre Way, Regeneration Medicine Building 903B
City San Francisco
State/province California
ZIP/Postal code 94143
Country USA
 
Platform ID GPL21290
Series (2)
GSE110469 Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells. [RNA-Seq - ex vivo]
GSE110472 Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells.
Relations
BioSample SAMN08518232
SRA SRX3680693

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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