|
Status |
Public on Nov 22, 2019 |
Title |
AdultNaive4 |
Sample type |
SRA |
|
|
Source name |
Human PBMCs
|
Organism |
Homo sapiens |
Characteristics |
cell type: adult CD4+CD25-CD45RA+CD27+ naive T cells tissue: Human PBMCs age: 70 years Sex: Male
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were sorted by FACS, and total RNA was extracted from 250,000 cells using the Nucleospin RNA kit (Machery Nagel) Libraries were constructed using a standard Illumina protocol
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Description |
AN4
|
Data processing |
Fastq files were mapped to the genome using Bowtie 2 Read counts per gene was obtained using RSEM TMM normalization and differential expression analysis performed using EdgeR package for Bioconductor Genome_build: hg19 Supplementary_files_format_and_content: TMM-normalized data.
|
|
|
Submission date |
Feb 12, 2018 |
Last update date |
Nov 24, 2019 |
Contact name |
Melissa Ng |
E-mail(s) |
melissa.ng@ucsf.edu
|
Organization name |
University of California, San Francisco
|
Lab |
Burt Lab
|
Street address |
35 Medical Centre Way, Regeneration Medicine Building 903B
|
City |
San Francisco |
State/province |
California |
ZIP/Postal code |
94143 |
Country |
USA |
|
|
Platform ID |
GPL21290 |
Series (2) |
GSE110469 |
Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells. [RNA-Seq - ex vivo] |
GSE110472 |
Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells. |
|
Relations |
BioSample |
SAMN08518244 |
SRA |
SRX3680681 |