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Sample GSM2947889 Query DataSets for GSM2947889
Status Public on Jan 26, 2018
Title NBL-WN_Normoxia_RNA-seq
Sample type SRA
 
Source name NBL-WN cells
Organism Homo sapiens
Characteristics cell line: NBL-WN
treatment: Treatment: 48 hours Normoxia
Treatment protocol 1% O2 (hypoxia) or 20% O2 (normoxia) for 48 hours
Growth protocol DMEM with 10% FBS
Extracted molecule total RNA
Extraction protocol RNA extracted with Trizol reagent.
Standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description processed data file:
WN_RNA-seq_Normoxia_vs_Hypoxia.txt
Data processing RNA-Seq: Reads aligned with tophat2 and bowtie2
RNA-Seq: Cufflinks (cufflinks, cuffcompare, cuffdiff) for expression analysis
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited output of FPKM for RNA
 
Submission date Jan 25, 2018
Last update date Jan 26, 2018
Contact name Christopher Mariani
E-mail(s) christopher.mariani@gmail.com
Organization name University of Chicago
Street address 900 E 57 St
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
 
Platform ID GPL16791
Series (1)
GSE55391 TET1-mediated hydroxymethylation facilitates hypoxic gene induction in neuroblastoma
Relations
BioSample SAMN08395452
SRA SRX3599592

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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