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Sample GSM2945918 Query DataSets for GSM2945918
Status Public on Nov 02, 2018
Title Species_mix_open-surface_format
Sample type SRA
Source name cell lines
Organisms Homo sapiens; Mus musculus
Characteristics cell type: NIH3T3 mouse fibroblast, K562 erythroleukemic cell line
passages: 5-8
Extracted molecule total RNA
Extraction protocol mRNA captured on barcoded beads were reverse transcribed and amplified using half a PCR reaction. Tagmentation and final library preparation was performed using Illumina Nextera XT Kit (FC-131-1024) according to manufacturer's protocol.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Data processing Base calling, alignment, filtering and generation of gene expression matrices are all performed according to the protocols described in Macosko et al. Cell 2015
Genome_build: hg19, mm10
Supplementary_files_format_and_content: tab limited text files include transcript counts for each gene for individual cells
Submission date Jan 23, 2018
Last update date Nov 03, 2018
Contact name Burak Dura
Phone 6173350782
Organization name Yale University
Department Biomedical Engineering
Lab Rong Fan Lab
Street address 55 Prospect St
City New Haven
State/province Connecticut
ZIP/Postal code 06511
Country USA
Platform ID GPL22245
Series (1)
GSE109535 scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3’ mRNA profiling
BioSample SAMN08388576
SRA SRX3593664

Supplementary file Size Download File type/resource
GSM2945918_Species_mix_open-surface_format.xlsx 59.8 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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