|
| Status |
Public on Apr 25, 2019 |
| Title |
MCF7 p21 rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
breast cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
treatment: ectopic express p21
|
| Treatment protocol |
The cells were irridated with 6 Gy or ectopic express p21
|
| Growth protocol |
MCF7 cells grow in DMEM mediu with 4mM glutamine and 10% Tet system approved FBS and penicillin-streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina™ TotalPrep™ RNA Amplification Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
p21 rep2
|
| Data processing |
The data were normalised using quantile normalisation with GenomeStudio. The filtering is detection p-value less than 0.05.
|
| |
|
| Submission date |
Jan 08, 2018 |
| Last update date |
Apr 25, 2019 |
| Contact name |
Stephen Kron |
| Organization name |
University of Chicago
|
| Department |
MGCB
|
| Lab |
Stephen Kron
|
| Street address |
929 E. 57th St, W519H
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE108895 |
Genome-wide analysis of senecent MCF7 cells induced by irridiation and ectopic overexpression of p21 |
|
