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Sample GSM291500 Query DataSets for GSM291500
Status Public on Jul 01, 2008
Title TotalRNA_0min_DMSO_E
Sample type RNA
 
Source name 0 min + DMSO
Organism Mus musculus
Characteristics NIH3T3 cell
Treatment protocol Serum starvation 36 h, 0 min + control DMSO
Growth protocol NIH3T3 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% calf serum.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a Qiagen RNeasy kit according to the manufacturer’s protocol. The rest of the protocols were adopted from Affymetrix WT double-stranded target assay manual (SuperScript III RT at 50 °C instead of SuperScript II RT at 42°C).
Label Biotin
Label protocol Standard Affymetrix protocol (WT double stranded DNA terminal labeling)
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description RNA mapping with total RNA, 0 min + DMSO, Affymetrix Mouse Tiling 2.0R E array
Data processing Bar files were created using Affymetrix Tiling SDK release2 (BPMAP = NCBIv35, Quantile normalization, Bandwidth = 250, Signal scale = log2).
 
Submission date May 28, 2008
Last update date May 28, 2008
Contact name Eisuke Nishida
E-mail(s) nishida@lif.kyoto-u.ac.jp
Phone +81-75-753-4230
Organization name Graduate School of Biostudies, Kyoto University
Department Department of Cell and Developmental Biology
Street address Kitashirakawa, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8502
Country Japan
 
Platform ID GPL6448
Series (1)
GSE11576 RNA mapping and ChIP with tiling arrays in FGF-stimulated NIH3T3 cells

Supplementary file Size Download File type/resource
GSM291500_TotalRNA_0min_DMSO_E.CEL.gz 23.0 Mb (ftp)(http) CEL
Processed data provided as supplementary file

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