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Sample GSM2911323 Query DataSets for GSM2911323
Status Public on Dec 27, 2020
Title 0826 OG2MEF
Sample type SRA
 
Source name fibroblasts
Organism Mus musculus
Characteristics strain: 129/OG2
cell type: fibroblasts
passages: 2
treatment: no
treatment time: 0 days
Extracted molecule total RNA
Extraction protocol RNA of cells was harvested using Trizol reagent on ice. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
We used standard Illumina protocols for sequencing to construct RNA libraries.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
 
Data processing Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie
Transcripts Per Million were calculated using a protocol from RSEM
Genome_build: mm10
Supplementary_files_format_and_content: TPM
 
Submission date Jan 03, 2018
Last update date Dec 27, 2020
Contact name zheng hui
Organization name GIBH
Street address Kaiyuan av.190
City Guangzhou
ZIP/Postal code 510000
Country China
 
Platform ID GPL16417
Series (1)
GSE108695 TET1 regulates reprogramming with Vitamin C and relatively small molecules
Relations
BioSample SAMN08287241
SRA SRX3527723

Supplementary file Size Download File type/resource
GSM2911323_0826OG2MEF.genes.txt.gz 718.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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