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Status |
Public on Dec 27, 2020 |
Title |
0826 OG2MEF |
Sample type |
SRA |
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Source name |
fibroblasts
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Organism |
Mus musculus |
Characteristics |
strain: 129/OG2 cell type: fibroblasts passages: 2 treatment: no treatment time: 0 days
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Extracted molecule |
total RNA |
Extraction protocol |
RNA of cells was harvested using Trizol reagent on ice. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. We used standard Illumina protocols for sequencing to construct RNA libraries.
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina MiSeq |
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Data processing |
Illumina Casava1.7 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie Transcripts Per Million were calculated using a protocol from RSEM Genome_build: mm10 Supplementary_files_format_and_content: TPM
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Submission date |
Jan 03, 2018 |
Last update date |
Dec 27, 2020 |
Contact name |
zheng hui |
Organization name |
GIBH
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Street address |
Kaiyuan av.190
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City |
Guangzhou |
ZIP/Postal code |
510000 |
Country |
China |
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Platform ID |
GPL16417 |
Series (1) |
GSE108695 |
TET1 regulates reprogramming with Vitamin C and relatively small molecules |
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Relations |
BioSample |
SAMN08287241 |
SRA |
SRX3527723 |
Supplementary file |
Size |
Download |
File type/resource |
GSM2911323_0826OG2MEF.genes.txt.gz |
718.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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