|
| Status |
Public on Dec 27, 2020 |
| Title |
0824 Ips D8 Flag DMSO |
| Sample type |
SRA |
| |
|
| Source name |
cells during reprogramming induced by OKSM
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129/OG2
cell type: cells during reprogramming induced by OKSM
passages: 0
treatment: DMSO
treatment time: 8 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA of cells was harvested using Trizol reagent on ice. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
We used standard Illumina protocols for sequencing to construct RNA libraries.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie
Transcripts Per Million were calculated using a protocol from RSEM
Genome_build: mm10
Supplementary_files_format_and_content: TPM
|
| |
|
| Submission date |
Jan 03, 2018 |
| Last update date |
Dec 27, 2020 |
| Contact name |
zheng hui |
| Organization name |
GIBH
|
| Street address |
Kaiyuan av.190
|
| City |
Guangzhou |
| ZIP/Postal code |
510000 |
| Country |
China |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
| GSE108695 |
TET1 regulates reprogramming with Vitamin C and relatively small molecules |
|
| Relations |
| BioSample |
SAMN08287228 |
| SRA |
SRX3527713 |
