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Sample GSM2891905 Query DataSets for GSM2891905
Status Public on Feb 12, 2018
Title L136-d86
Sample type SRA
 
Source name Seminal exosomes
Organism Equus caballus
Characteristics infection: Equiena arteritis virus
time: 86 days post-infection
Treatment protocol Seminal exosomes were isolated using the ExoQuick Exosome Precipitation Solution (System Biosciences, Palo Alto, CA) from seminal plasma samples derived from the stallions at the specific time-points post-infection as described
Extracted molecule total RNA
Extraction protocol Exosomal RNA was extracted using the SeraMir Exosome RNA Isolation kit (System Biosciences, Palo Alto, CA)
cDNA libraries were prepared using TailorMix Micro RNA Sample Preparation version 2 (SeqMatic LLC, Fremont, CA) and perfomed by a commercial company (System Biosciences, Palo Alto, CA). cDNA libraries were size-selected on an 8% Tris-borate-EDTA (TBE) polyacrylamide gel
cDNA libraries were sequenced (1x75bp) in a Illumina NextSeq 500 platform.
 
Library strategy miRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina NextSeq 500
 
Data processing bcl2Fastq
TrimGalore
mirDeep2
Genome_build: GCA_000002305.1
Supplementary_files_format_and_content: miRDeep2 csv result output
 
Submission date Dec 18, 2017
Last update date Feb 12, 2018
Contact name Theodore Samuel Kalbfleisch
E-mail(s) ted.kalbfleisch@louisville.edu
Phone 502-852-7495
Organization name University of Louisville
Department Biochemistry and Molecular Genetics
Lab Kalbfleisch
Street address 221F Baxter II, 580 S Preston St
City Louisville
State/province KY
ZIP/Postal code 40202
Country USA
 
Platform ID GPL21401
Series (1)
GSE108180 Downregulation of eca-mir-128 in seminal exosomes and enhanced expression of CXCL16 in the stallion reproductive tract are associated with equine arteritis virus long-term persistent infection
Relations
BioSample SAMN08196564
SRA SRX3478264

Supplementary file Size Download File type/resource
GSM2891905_result_L136-d86.csv.gz 30.0 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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