|
Status |
Public on Feb 12, 2018 |
Title |
L136-pre |
Sample type |
SRA |
|
|
Source name |
Seminal exosomes
|
Organism |
Equus caballus |
Characteristics |
infection: Uninfected time: Pre-challenge
|
Treatment protocol |
Seminal exosomes were isolated using the ExoQuick Exosome Precipitation Solution (System Biosciences, Palo Alto, CA) from seminal plasma samples derived from the stallions at the specific time-points post-infection as described
|
Extracted molecule |
total RNA |
Extraction protocol |
Exosomal RNA was extracted using the SeraMir Exosome RNA Isolation kit (System Biosciences, Palo Alto, CA) cDNA libraries were prepared using TailorMix Micro RNA Sample Preparation version 2 (SeqMatic LLC, Fremont, CA) and perfomed by a commercial company (System Biosciences, Palo Alto, CA). cDNA libraries were size-selected on an 8% Tris-borate-EDTA (TBE) polyacrylamide gel cDNA libraries were sequenced (1x75bp) in a Illumina NextSeq 500 platform.
|
|
|
Library strategy |
miRNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
bcl2Fastq TrimGalore mirDeep2 Genome_build: GCA_000002305.1 Supplementary_files_format_and_content: miRDeep2 csv result output
|
|
|
Submission date |
Dec 18, 2017 |
Last update date |
Feb 12, 2018 |
Contact name |
Theodore Samuel Kalbfleisch |
E-mail(s) |
ted.kalbfleisch@louisville.edu
|
Phone |
502-852-7495
|
Organization name |
University of Louisville
|
Department |
Biochemistry and Molecular Genetics
|
Lab |
Kalbfleisch
|
Street address |
221F Baxter II, 580 S Preston St
|
City |
Louisville |
State/province |
KY |
ZIP/Postal code |
40202 |
Country |
USA |
|
|
Platform ID |
GPL21401 |
Series (1) |
GSE108180 |
Downregulation of eca-mir-128 in seminal exosomes and enhanced expression of CXCL16 in the stallion reproductive tract are associated with equine arteritis virus long-term persistent infection |
|
Relations |
BioSample |
SAMN08196566 |
SRA |
SRX3478261 |