GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM2861420 Query DataSets for GSM2861420
Status Public on Jan 30, 2018
Title 5wo male #2
Sample type SRA
Source name Thymus
Organism Mus musculus
Characteristics cell type: CD19+ thymic B cells
age: 5 weeks
strain: C57BL/6J
Treatment protocol Thymocytes were stained with CD19-APC, tagged with anti-APC magnetic beads (Miltenyi Biotec) and magnetically enriched. CD19+ were sorted from enriched samples using BD FACS Aria to a minimum purity of 98.5% or better.
Growth protocol Animals kept in SPF conditions
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from sorted B cells using Invitrogen RNAaqueous micro kit
RNA libraries were prepared using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Description Young male
Data processing Illumina Casava v1.8.2 software used for base-calling
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm9 whole genome using TopHat v2.0.8b
gene count measurements were obtained using HTSeq-count
Genome_build: UCSC mm9
Supplementary_files_format_and_content: The processed data files are in tabular format. The first column contains the gene symbols and the second column contain the read counts of the gene using htseq-count
Submission date Nov 17, 2017
Last update date May 15, 2019
Contact name Yidong Chen
Phone 2105629163
Organization name UT Health Science Center at San Antonio
Department Population Health Sciences
Street address 8403 Floyd Curl Drive, MSC 7784
City San Antonio
State/province Texas
ZIP/Postal code 78229
Country USA
Platform ID GPL21493
Series (2)
GSE107110 RNA-sequencing of bulk CD19+ Thymic B cells from young (5 week) and aged (10-24 month) mice
GSE107112 RNA-sequencing of bulk CD19+ Thymic B cells from mice and humans
SRA SRX3405798
BioSample SAMN08045303

Supplementary file Size Download File type/resource
GSM2861420_Sample_Bcells_5wo_male_2_Genes_ReadCount.txt.gz 123.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap