|
Status |
Public on Nov 06, 2017 |
Title |
RO_RANKL_Day3_Rep1 |
Sample type |
SRA |
|
|
Source name |
Intestinal organoid epithelial cells
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 treatment: treated with RANKL (100 ng ml−1) time point: 3 days after RANKL treatment genotype: Wild-type
|
Extracted molecule |
total RNA |
Extraction protocol |
3' polyA RNA seq 10X Genomics Chromium Prep
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
3' polyA RNA-seq CellRanger 1.0 PCA (prcomp in R) kNN-based graph clustering (R code) Genome_build: mm10 Supplementary_files_format_and_content: 3' polyA RNA-seq. The expression levels for each gene (rows) in each single cell (column) in UMI counts as calculated using the CellRanger pipeline. Each column header provides the sequencing batch id, the cell barcode, and the cell type as identified by cluster analysis. Each ID is seperated by '_'
|
|
|
Submission date |
Nov 03, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Adam Haber |
E-mail(s) |
ahaber@hsph.harvard.edu
|
Organization name |
Harvard T. H. Chan School of Public Health
|
Street address |
665 Huntington Ave, HSPH Building 1, Room 305
|
City |
Boston |
State/province |
Massachusetts |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE92332 |
A single-cell survey of the small intestinal epithelium |
|
Relations |
BioSample |
SAMN07976248 |
SRA |
SRX3361144 |