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Sample GSM2831508 Query DataSets for GSM2831508
Status Public on Dec 20, 2017
Title tumor from HN1L siRNA-treated mouse #4814 [miRNA]
Sample type RNA
 
Source name patient-derived xenograft tumor from HN1L siRNA-treated mouse #4814
Organism Homo sapiens
Characteristics tissue: breast cancer tumor tissue
genotype: Triple-negative breast cancer
xenograft type: BCM2665
Treatment protocol Groups (0) and (1) (n=10) were treated with 5 µg/mouse DOPC nanoliposomal siRNA IP injection twice a week for 3 weeks. Mice were sacrificed on day 21. Tumors were harvested and snap frozen.
Growth protocol In docetaxel-resistant BCM2665 PDX xenografts, tumors were transplanted into the mammary fat pad of SCID-Beige mice. Mice were randomized into 5 groups when tumor volume reached 150-200mm3: (0)scrambled siRNA, (1)HN1L siRNA.
Extracted molecule total RNA
Extraction protocol Extraction of miRNA from each frozen tumor sample was performed using miRNeasy Mini kit from Qiagen following manufacturer instruction.
Label biotin
Label protocol Biotinylated miRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol hybridization,washing and staining were done following Affymetrix protocol for Genechip miRNA 4.0 arrays.
Scan protocol Scanning was done on Affymetrix Genechip Scanner model 7G, Affymetrix Genechip command console(AGCC) software was used for instrument control, data acquisition and quality control.
Description miRNA expression data from TNBC tumor sample of HN1L-siRNA treated mouse model BCM2665
Data processing Expression values were processed and normalized using the affy library in R statistical software. Only human probes were isolated, from the multispecies miRNA profiling by the miRNA array 4.0, for the purpose of the analysis. Differential expression analysis was then performed using limma package in R. The most significantly differentially expressed miRNAs were selected based on a cut off log fold change > 1 and FDR < 0.2.
 
Submission date Oct 26, 2017
Last update date Jan 23, 2018
Contact name Jenny Chang
Organization name Houston Methodist Hospital
Department Houston Methodist Cancer Center
Street address 6670 Bertner Ave.
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL19117
Series (2)
GSE106199 MicroRNA differencial expression data between scrambled siRNA-treated mice and HN1L siRNA-treated mice tumor samples
GSE106200 RNA differencial expression data between scrambled siRNA-treated mice and HN1L siRNA-treated mice tumor samples

Data table header descriptions
ID_REF
VALUE Values were processed and normalized using the affy library in R statistical software, presented as normalized log2 expression value

Data table
ID_REF VALUE
MIMAT0021116_st 0.987989881
MIMAT0021117_st 2.883252326
MIMAT0021118_st 0.664089515
MIMAT0021119_st 0.654587528
MIMAT0021120_st 8.518740217
MIMAT0027088_st 1.071044239
MIMAT0021121_st 2.180606497
MIMAT0021122_st 1.028934529
MIMAT0019853_st 4.748867434
MIMAT0019854_st 1.193344871
MIMAT0019855_st 7.207612775
MIMAT0019856_st 0.724879913
MIMAT0019857_st 1.058634384
MIMAT0019858_st 1.153667289
MIMAT0019859_st 12.41553363
MIMAT0019860_st 0.664089515
MIMAT0019861_st 0.833651435
MIMAT0019862_st 0.997013479
MIMAT0019863_st 0.773497927
MIMAT0019864_st 7.699723954

Total number of rows: 6631

Table truncated, full table size 178 Kbytes.




Supplementary file Size Download File type/resource
GSM2831508_1_4814_miRNA-4_0_.CEL.gz 828.6 Kb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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