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Sample GSM282126 Query DataSets for GSM282126
Status Public on Nov 24, 2008
Title WTGR(Dex)::3
Sample type RNA
 
Source name WT Glucocorticoid receptor alpha treated for 6 hr, replicate 3
Organism Homo sapiens
Characteristics Human osteosarcoma, female
Extracted molecule total RNA
Extraction protocol The RNeasy RNA extraction kit, with on column DNAse digestion (Qiagen) was used, according to manufacturer's protocol.
Label Cy3
Label protocol Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
 
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slides were washed and then scanned with an Agilent Scanner.
Description Cells were grown in DMEM-F12 medium, supplemented with 10% Fetal Bovine Serum. 48 hour before treatments, cells were placed in DMEM-F12 medium supplemented with 10% charcoal-dextran-treated FBS. Cells were not treated or were treated with 100 nM dexamethasone for 6 hr.
Data processing Data were obtained and processed with the Agilent Feature Extraction Software (v9.5). The Agilent Feature Extraction Software performed error modeling, adjusting for additive and multiplicative noise. The resulting data were imported into Rosetta Resolver (v7.1) and log2 intensities were exported.
 
Submission date Apr 17, 2008
Last update date Jan 05, 2012
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL4133
Series (1)
GSE11205 Glycogen Synthase Kinase-3-beta Regulates Glucocorticoid Signaling by Phosphorylating the Glucocorticoid Receptor

Data table header descriptions
ID_REF Agilent Feature Number
VALUE Log2 intensity exported from Rosetta Resolver

Data table
ID_REF VALUE
44919 11.49405
8563 4.28067
28336 6.71123
5006 15.55935
28801 14.38422
29429 16.31158
38756 9.35956
31922 13.74553
3509 2.60947
27587 12.14299
18581 8.45949
10122 10.08972
14308 5.34641
35097 4.51532
22537 3.27699
1387 11.23281
41336 8.85911
33919 2.60297
24114 14.52572
9363 10.56846

Total number of rows: 40999

Table truncated, full table size 560 Kbytes.




Supplementary file Size Download File type/resource
GSM282126.txt.gz 8.9 Mb (ftp)(http) TXT
GSM282126_US22502625_251485022325_S01_H.tif.gz 61.5 Mb (ftp)(http) TIFF
GSM282126_US22502625_251485022325_S01_L.tif.gz 46.4 Mb (ftp)(http) TIFF
Processed data included within Sample table

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