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Sample GSM282123 Query DataSets for GSM282123
Status Public on Nov 24, 2008
Title WTGR(-)::3
Sample type RNA
Source name WT Glucocorticoid receptor alpha treated for 0 hr, replicate 3
Organism Homo sapiens
Characteristics Human osteosarcoma, female
Extracted molecule total RNA
Extraction protocol The RNeasy RNA extraction kit, with on column DNAse digestion (Qiagen) was used, according to manufacturer's protocol.
Label Cy3
Label protocol Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slides were washed and then scanned with an Agilent Scanner.
Description Cells were grown in DMEM-F12 medium, supplemented with 10% Fetal Bovine Serum. 48 hour before treatments, cells were placed in DMEM-F12 medium supplemented with 10% charcoal-dextran-treated FBS. Cells were not treated or were treated with 100 nM dexamethasone for 6 hr.
Data processing Data were obtained and processed with the Agilent Feature Extraction Software (v9.5). The Agilent Feature Extraction Software performed error modeling, adjusting for additive and multiplicative noise. The resulting data were imported into Rosetta Resolver (v7.1) and log2 intensities were exported.
Submission date Apr 17, 2008
Last update date Jan 05, 2012
Contact name NIEHS Microarray Core
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
Platform ID GPL4133
Series (1)
GSE11205 Glycogen Synthase Kinase-3-beta Regulates Glucocorticoid Signaling by Phosphorylating the Glucocorticoid Receptor

Data table header descriptions
ID_REF Agilent Feature Number
VALUE Log2 intensity exported from Rosetta Resolver

Data table
44919 11.67082
8563 4.93543
28336 7.24645
5006 15.21444
28801 14.16816
29429 16.52575
38756 8.5455
31922 13.461
3509 2.57342
27587 12.50495
18581 8.50387
10122 10.26279
14308 5.55469
35097 3.38283
22537 4.07827
1387 10.17949
41336 9.23877
33919 3.51706
24114 14.27465
9363 9.24839

Total number of rows: 41000

Table truncated, full table size 560 Kbytes.

Supplementary file Size Download File type/resource
GSM282123.txt.gz 8.9 Mb (ftp)(http) TXT
GSM282123_US22502625_251485022324_S01_H.tif.gz 62.1 Mb (ftp)(http) TIFF
GSM282123_US22502625_251485022324_S01_L.tif.gz 47.1 Mb (ftp)(http) TIFF
Processed data included within Sample table

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