|
| Status |
Public on May 30, 2018 |
| Title |
CD8αβ+ SPLs, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
CD8αβ+ SPLs, replicate 1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
gender: male
|
| Treatment protocol |
CD8αβ+ IELs and CD8αβ+ SPLs were sorted from intraepithelium of small intestines and spleens of WT mice (8–12w), and we lysed the cells in TRIzol (Invitrogen) and freezed them in refrigerator (-80°C).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using miRNeasy Mini Kit and RNase-Free Dnase Set.Total RNA quality was assessed with a Pico Chip on an Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
indocarbocyanine
|
| Label protocol |
RNA was amplified and abeling of CTP with indocarbocyanine was done with Agilent one-color labeling kits (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Indocarbocyanine-labeled cDNA was assessed with the NanoDrop ND-2000 (Thermo Scientific), and equal amounts of indocarbocyanine-labeled target were hybridized to Agilent SurePrint G3 Mouse Gene Expression (8x60K) microarrays（Design ID：74809）.
|
| Scan protocol |
Arrays were scanned with the Agilent Scanner G2505C(Agilent Technologies).
|
| Data processing |
Raw signal intensities were extracted with Feature Extraction v10.7.1.1 software(Agilent Technologies).
|
| |
|
| Submission date |
Oct 17, 2017 |
| Last update date |
May 30, 2018 |
| Contact name |
Banru Chen |
| E-mail(s) |
chenbr@mail.ustc.edu.cn
|
| Organization name |
School of Life Sciences, University of Science & Technology of China
|
| Street address |
443 Huangshan Road
|
| City |
Hefei |
| ZIP/Postal code |
230027 |
| Country |
China |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE105061 |
Gene expression signatures for CD8αβ+ intraepithelial lymphocytes and CD8αβ+ splenocytes. |
|
