|
| Status |
Public on Oct 17, 2017 |
| Title |
WASH3 |
| Sample type |
RNA |
| |
|
| Source name |
T6E cells, GSI washoutt
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
cell line: T6E
|
| Treatment protocol |
T6E cells were treated with either DMSO or GSI (1 μM) containing medium for 24 hours. Subsequently, cells were cultured in media containing cycloheximide (20 μg/mL) and cells previously cultured DMSO were replated in DMSO (lebeled DMSO) and cells cultured in GSI were either replated in 1 μM GSI (labeled GSI) or in DMSO (labeled WASH) for 6 hours.
|
| Growth protocol |
T6E cells were cultured in RPMI (Life Technologies) medium supplemented with 10% heat-inactivated FBS (Hyclone), 100 U/ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine, 50 mM 2-mercaptoethanol,
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the QIAGEN RNeasy mini Kit.
|
| Label |
biotin
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
not provided
|
| Scan protocol |
not provided
|
| Description |
Gene expression data from T6E cells treated with GSI for 24h and then treatment was washed out and cells were cultured with DMSO for 6h.
|
| Data processing |
data is processed with biocondocutor oligo package and background correcting and rma normalized
|
| |
|
| Submission date |
Oct 16, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Robert Babak Faryabi |
| E-mail(s) |
faryabi@pennmedicine.upenn.edu
|
| Phone |
215-573-8220
|
| Organization name |
University of Pennsylvania
|
| Department |
Pathology
|
| Lab |
Faryabi Lab
|
| Street address |
Room 553 BRB II/III, 421 Curie Boulevard
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE104991 |
MafB enhances oncogenic Notch signaling in T-ALL [Microarray] |
| GSE104993 |
MafB enhances oncogenic Notch signaling in T-ALL |
|
