|
| Status |
Public on Jul 22, 2008 |
| Title |
Histone AcH4 ChIP-chip data of Wild Type |
| Sample type |
genomic |
| |
|
| Source name |
Distribution of Histone AcH4 in wild type.
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
Chromatin was prepared from wild type S.pombe cells. Chromatin-IP was carried out by pan Ac-H4 antibody.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
We disrupted 1.5 x 10^8 cells byMulti-beads shocker (MB400U, Yasui Kikai) using glass beads. Anti-pan Ac-H4(Upstate)was used for chromatin immunoprecipitation.
|
| Label |
Biotin-11-ddATP
|
| Label protocol |
DNA was amplified by IVT (In vitro transcription) method. For the labeling, DNA was fragmented by DNaseI. DNA was end-labeled by Terminal Transferase with biotin‐N11‐ddATP (NEL508).
|
| |
|
| Hybridization protocol |
Each sample was hybridized to the array in 200 ul containing 1XHybridization buffer (Affymetrix), 50pM Control oligonucleotide (oligo B2, Affymetrix), Herring Sperm DNA (0.1mg/ml), Acetylated BSA (0.5mg/ml), and 7% DMSO. Samples were denatured at 100C for 10 minutes, and then put on ice before being hybridized for 16 hours at 45C in an hybridization oven (GeneChip Hybridization Oven 640, Affymetrix). Washing and scanning protocol provided by Affymetrix was performed automatically on a fluidics station (GeneChip fluidics station 450, Affymetrix).
|
| Scan protocol |
Arrays were scanned using the Genechip Scanner3000 7G following the library array description.
|
| Description |
Histone AcH4 binding profile of Wild Type.
|
| Data processing |
Data was processed following the protocol provided by Affymetrix (GCOS ver 1.4).
|
| |
|
| Submission date |
Apr 09, 2008 |
| Last update date |
Jul 22, 2008 |
| Contact name |
Katsuhiko Shirahige |
| E-mail(s) |
kshirahi@iam.u-tokyo.ac.jp
|
| Phone |
+81-3-5842-0756
|
| Fax |
+81-3-5842-0757
|
| URL |
http://www.iam.u-tokyo.ac.jp/chromosomeinformatics/
|
| Organization name |
The University of Tokyo
|
| Department |
Research Center for Epigenetic Disease
|
| Lab |
Laboratory of Genome Structure and Function
|
| Street address |
1-1-1 Yayoi
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-0032 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3847 |
| Series (1) |
| GSE11102 |
A DNA polymerase α accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast |
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