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Sample GSM279939 Query DataSets for GSM279939
Status Public on May 28, 2008
Title MCV8_H3K27me3_ChIPSeq
Sample type genomic
 
Source name MEF-derived cell line MCV8
Organism Mus musculus
Characteristics Consititutive Oct4, Klf4, c-Myc, Sox2 retrovirus
Antibody: H3K27me3 (Upstate 07-449)
Growth protocol MCV6, MCV8, MCV8.1 and BIV1 were cultured under standard mouse ES cell contitions in the prescence of LIF (see Mikkelsen, 2008)
Extracted molecule genomic DNA
Extraction protocol Described in Mikkelsen et al., Nature 2007. Briefly, chromatin was fixed in 1% formaldehyde, sheared to 200-700bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.
 
Description Antibody: H3K27me3 (Upstate 07-449)
Data processing Described in Mikkelsen et al, 2008. Briefly, sequence reads from each IP experiment were aligned to the mouse reference genome (mm8), and all uniquely aligned reads (within >2 mismatches) were kept. Read densities were computed by counting the number of reads (extended to 300 bp) overlapping each position in the genome (at 25bp resolution).
 
Submission date Apr 05, 2008
Last update date May 15, 2019
Contact name Tarjei S Mikkelsen
Organization name Broad Institute
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL6693
Series (1)
GSE11074 Chromatin state maps (H3K4me3 and H3K27me3) from partially and fully reprogrammed mouse cell lines
Relations
SRA SRX000418

Supplementary file Size Download File type/resource
GSM279939_MCV8.H3K27me3.aligned.txt.gz 215.6 Mb (ftp)(http) TXT
GSM279939_MCV8.H3K27me3.densities.txt.gz 280.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data provided as supplementary file

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