NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2777159 Query DataSets for GSM2777159
Status Public on Sep 22, 2017
Title control 24h-exp2
Sample type RNA
 
Source name Human primary fibroblasts transduced by empty viruses for 24h
Organism Homo sapiens
Characteristics cell line: human lung normal fibroblast, ATCC CCL-75
gender: female
developmental stage: 3 months gestation fetus
ethnicity: Caucasian
genotype/variation: control
time: 24h
Extracted molecule total RNA
Extraction protocol RNA samples were extracted from fresh transduced cells, with Rneasy Mini Kit, according to the manufacturer’s instructions. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA. A 2-round amplification was performed with the ExpressArt TRinucleotide mRNA Amplification Kit (AmpTec) according to the manufacturer’s instructions.
Label biotin
Label protocol 100ng of total RNA were amplified to generate cRNA; 10µg of cRNA were converted into single stranded DA, based on the WT cDNA Synthesis Kit (Affymetrix). 5,5µg of cDNA were fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized onto GeneChip Human Gene 2.1 ST Arrays (Affymetrix) using the GeneTitan platform at the Genomics platform, Translational Resarch Dept, Institut Curie , Paris, France. 3.3µg of targets were hybridized according Affymetrix recommendations.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description Gene expression data from transduced human primary cells using Affy Human Gene v2.1
Data processing Raw data were processed with Expression Console from Affymetrix. A RMA normlization was applied to control to data.
Filtering details no longer available.
 
Submission date Sep 08, 2017
Last update date Jan 23, 2018
Contact name Michiko Kawakita
E-mail(s) rinko.kawa@gmail.com
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
 
Platform ID GPL17692
Series (2)
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress
GSE103642 PML is a ROS sensor activating p53 upon oxidative stress

Data table header descriptions
ID_REF
VALUE unlogged, normalized intensities using GcRMA

Data table
ID_REF VALUE
16657450 320.55
16657469 90.17
16657476 109.03
16657514 124.82
16657534 93.14
16657554 451.37
16657594 301.65
16657598 164.77
16657680 138.89
16657713 78.26
16657730 166.53
16657821 116.97
16657856 123.68
16657910 519.67
16657993 814.70
16658005 80.25
16658106 583.52
16658184 70.20
16658231 236.91
16658449 430.02

Total number of rows: 11869

Table truncated, full table size 184 Kbytes.




Supplementary file Size Download File type/resource
GSM2777159_EX41_ARN0025_s1h1_A05_hGene_2.1.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap