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Sample GSM2777154 Query DataSets for GSM2777154
Status Public on Sep 22, 2017
Title PML 4 18h-exp1
Sample type RNA
Source name Human primary fibroblasts transduced by PML4 expressing viruses for 18h
Organism Homo sapiens
Characteristics cell line: human lung normal fibroblast, ATCC CCL-75
gender: female
developmental stage: 3 months gestation fetus
ethnicity: Caucasian
genotype/variation: PML4
time: 18h
Extracted molecule total RNA
Extraction protocol RNA samples were extracted from fresh transduced cells, with Rneasy Mini Kit, according to the manufacturer’s instructions. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA. A 2-round amplification was performed with the ExpressArt TRinucleotide mRNA Amplification Kit (AmpTec) according to the manufacturer’s instructions.
Label biotin
Label protocol 100ng of total RNA were amplified to generate cRNA; 10µg of cRNA were converted into single stranded DA, based on the WT cDNA Synthesis Kit (Affymetrix). 5,5µg of cDNA were fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized onto GeneChip Human Gene 2.1 ST Arrays (Affymetrix) using the GeneTitan platform at the Genomics platform, Translational Resarch Dept, Institut Curie , Paris, France. 3.3µg of targets were hybridized according Affymetrix recommendations.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description Gene expression data from transduced human primary cells using Affy Human Gene v2.1
Data processing Raw data were processed with Expression Console from Affymetrix. A RMA normlization was applied to control to data.
Filtering details no longer available.
Submission date Sep 08, 2017
Last update date Jan 23, 2018
Contact name Michiko Kawakita
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
Platform ID GPL17692
Series (2)
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress
GSE103642 PML is a ROS sensor activating p53 upon oxidative stress

Data table header descriptions
VALUE unlogged, normalized intensities using GcRMA

Data table
16657450 267.28
16657469 95.12
16657476 64.54
16657514 130.38
16657534 118.62
16657554 115.79
16657594 85.99
16657598 221.43
16657680 160.42
16657713 189.47
16657730 76.97
16657821 277.86
16657856 122.03
16657910 143.80
16657993 591.91
16658005 767.45
16658106 77.39
16658184 495.13
16658231 68.01
16658449 290.95

Total number of rows: 11869

Table truncated, full table size 184 Kbytes.

Supplementary file Size Download File type/resource
GSM2777154_EX41_ARN0005_s2h1_E05_hGene2.1.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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