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Sample GSM2740590 Query DataSets for GSM2740590
Status Public on Feb 10, 2023
Title 4L-SCR_Input
Sample type SRA
 
Source name Melanoma cell line
Organism Homo sapiens
Characteristics cell type: WM239-derived 113/6-4L melanoma cell line
chip antibody: none (input)
Extracted molecule genomic DNA
Extraction protocol ChIP-IT High Sensitivity Kit (Active Motif), according the manufacturer recommendations.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
 
Data processing Base calling and demultiplexing was performed using Illumina bcl2fastq software.
Reads were aligned to the human genome (build hg19/GRCh37) with Bowtie 2 using local alignment.
Likely PCR duplicates were removed with Picard MarkDuplicates tool.
MACS was utilized to perform peak calling for each replicate.
Genome_build: hg19
Supplementary_files_format_and_content: BED files of peaks
 
Submission date Aug 11, 2017
Last update date Feb 10, 2023
Contact name Igor Dolgalev
Organization name NYU Grossman School of Medicine
Street address 550 1st Ave
City New York
State/province New York
ZIP/Postal code 10016
Country USA
 
Platform ID GPL16791
Series (2)
GSE102552 NR2F2 ChIP-sequencing of melanoma cell lines
GSE102554 NR2F2 melanoma cellular models
Relations
BioSample SAMN07499418
SRA SRX3088616

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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