|
| Status |
Public on Sep 24, 2017 |
| Title |
gr-con-2 |
| Sample type |
SRA |
| |
|
| Source name |
MCF10A_none (untreated control)_1 hour
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF10A
treated with: none (untreated control)
treatment time: 1 hour
chip antibody: rabbit anti-GR polyclonal antibody (Thermo Fisher Scientific, PA1-511A)
|
| Treatment protocol |
Cells were starved overnight in medium without additives and then stimulated with DEX (d) or the combination of epidermal growth factor (EGF) + DEX (ed) for the indicated time intervals (minutes) before sequencing
|
| Growth protocol |
MCF10A cells were cultured as described (Tarcic et al., 2012) in DME:F12 medium (Gibco BRL, Grand Island, NY) supplemented with 10 µg ml-1 insulin, 0.1 µg ml-1 cholera toxin, 0.5 µg ml-1 hydrocortison, 5% heat-inactivated horse serum (Biological Industries, Beit-Haemek, Israel) and 10 ng ml-1 EGF.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cell fixation and chromatin immunoprecipitation were performed as described (Blecher-Gonen et al., 2013)
Library construction and sequencing were performed as described (Blecher-Gonen et al., 2013)
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Chromatin immunoprecipitation (ChIP) sequencing reads were mapped to the hg19 genome assembly using Bowtie2 (Langmead and Salzberg, 2012)
Genome_build: hg19
Supplementary_files_format_and_content: Tag read densities were generated by the makeTagDirectory from the Homer suite
|
| |
|
| Submission date |
Aug 08, 2017 |
| Last update date |
Sep 26, 2019 |
| Contact name |
Yehoshua Enuka |
| E-mail(s) |
ys.enuka@gmail.com
|
| Phone |
+972-54-2345623
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Biological Regulation
|
| Lab |
Yarden
|
| Street address |
Herzl 234
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE94063 |
Epigenetic mechanisms underlie the crosstalk between growth factors and a steroid hormone |
| GSE102355 |
Epigenetic mechanisms underlie the crosstalk between growth factors and a steroid hormone [GR ChIP-seq] |
|
| Relations |
| BioSample |
SAMN07460486 |
| SRA |
SRX3070468 |
