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Sample GSM2728840 Query DataSets for GSM2728840
Status Public on May 22, 2019
Title Flag-KDM4B
Sample type SRA
 
Source name adipocyte, Flag-KDM4B retrovirus
Organism Mus musculus
Characteristics tissue: adipocyte
chip antibody: anti-Flag (M2, Sigma)
Growth protocol preadiocytes were isolated from Kdm4b KO mice, infected with control or Flag-KDM4B retrovirus and maintained with adipogenesis medium for 7 to 10 days
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated nuclei and KDM4B-DNA complexes were isolated with Flag antibody.
libaray was prepared according to KAPA Hyper prep kit
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
 
Data processing ChIP-seq reads were aligned to the mm9 genome assembly using Bowtie
peaks were called using algorithm described previously (Ferrari R et al., Genome Res. 2012:1212-21) with the following setting
Genome_build: mm9
 
Submission date Aug 02, 2017
Last update date May 22, 2019
Contact name Cunyu Wang
E-mail(s) cwang@dentistry.ucla.edu
Phone 3108256317
Organization name UCLA
Department School of Dentistry
Lab CHS 56-200
Street address 10833 Le Conte Ave
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL19057
Series (2)
GSE102148 KDM4B epigenetically protects against obesity and metabolic dysfunction [ChIP-seq]
GSE102290 KDM4B epigenetically protects against obesity and metabolic dysfunction
Relations
BioSample SAMN07437179
SRA SRX3055074

Supplementary file Size Download File type/resource
GSM2728840_Flag_Kdm4b.bed.gz 149.5 Kb (ftp)(http) BED
GSM2728840_Flag_Kdm4b_sigtags.wig.gz 58.0 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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