|
Status |
Public on May 22, 2019 |
Title |
Flag-KDM4B |
Sample type |
SRA |
|
|
Source name |
adipocyte, Flag-KDM4B retrovirus
|
Organism |
Mus musculus |
Characteristics |
tissue: adipocyte chip antibody: anti-Flag (M2, Sigma)
|
Growth protocol |
preadiocytes were isolated from Kdm4b KO mice, infected with control or Flag-KDM4B retrovirus and maintained with adipogenesis medium for 7 to 10 days
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Lysates were clarified from sonicated nuclei and KDM4B-DNA complexes were isolated with Flag antibody. libaray was prepared according to KAPA Hyper prep kit
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
ChIP-seq reads were aligned to the mm9 genome assembly using Bowtie peaks were called using algorithm described previously (Ferrari R et al., Genome Res. 2012:1212-21) with the following setting Genome_build: mm9
|
|
|
Submission date |
Aug 02, 2017 |
Last update date |
May 22, 2019 |
Contact name |
Cunyu Wang |
E-mail(s) |
cwang@dentistry.ucla.edu
|
Phone |
3108256317
|
Organization name |
UCLA
|
Department |
School of Dentistry
|
Lab |
CHS 56-200
|
Street address |
10833 Le Conte Ave
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE102148 |
KDM4B epigenetically protects against obesity and metabolic dysfunction [ChIP-seq] |
GSE102290 |
KDM4B epigenetically protects against obesity and metabolic dysfunction |
|
Relations |
BioSample |
SAMN07437179 |
SRA |
SRX3055074 |