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Sample GSM2719999 Query DataSets for GSM2719999
Status Public on Dec 03, 2017
Title RNA-seq_sham_d4_rep1
Sample type SRA
 
Source name Mouse left ventricle, sham day 4.
Organism Mus musculus
Characteristics tissue: Heart
Growth protocol Heart tissues were harvested at indicated time. Left ventricles were dissected and immediately frozen in liquid nitrogen.
Extracted molecule total RNA
Extraction protocol For RNA-seq analysis using the Illumina NextSeq500, 0.1-2 ug of total RNA was processed for library generation using the NuGEN Ovation RNA-seq library prep kit following the manufacturer’s protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description RNA-seq_read_count.txt
RNA-seq_RPKM.txt
Data processing Sequencing reads were aligned to human genome assembly hg19 by TopHat.
The number of reads aligned to RefSeq annotated genes' exons were counted and further normalized to be of unit RPKM (number of reads per kilo base pair per million total reads).
Differential gene expression analyses were performed using DEseq.
Genome_build: hg19
Supplementary_files_format_and_content: text file of gene read count values
Supplementary_files_format_and_content: text file of gene RPKM values
 
Submission date Jul 27, 2017
Last update date May 15, 2019
Contact name Jian Xu
E-mail(s) Jian.Xu@stjude.org
Phone 9015955208
Organization name St. Jude Children's Research Hospital
Department Pathology
Street address 262 Danny Thomas Place, MS 345
City Memphis
State/province Tennessee
ZIP/Postal code 38105
Country USA
 
Platform ID GPL19057
Series (1)
GSE101977 RNA-seq transcriptonal profiling in mouse hearts under pressure overload
Relations
BioSample SAMN07419971
SRA SRX3044463

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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