GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM2719983 Query DataSets for GSM2719983
Status Public on Jul 28, 2017
Title HGSC_JH1069
Sample type RNA
Source name primary ovarian tumor tissue
Organism Homo sapiens
Characteristics stic: No
age: 49
path.stage: IIC
surgical.outcome: Optimal
diagnosis.of.record: Ovarian Ca Ovarian Ca
platinumfreeinterval.mos..: 9
platinumstatus: Sensitive
recurrence: Y
pfs.mos..: 13.3
os.mos..: 60.2
vitalstatus: Alive
race: OTHER
tcga_subtype_dif: 0.41
tcga_subtype_imr: 0.144
tcga_subtype_mes: -0.22
tcga_subtype_pro: -0.275
tissue: primary tumor tissue from high grade serous ovarian tumors
Extracted molecule total RNA
Extraction protocol DNA and RNA were co-isolated from frozen tissue tumor blocks, and RNA was isolated from normal tissues utilizing Ambion’s ToTALLY RNA™ RNA Isolation Kit (Part Number AM1910, ©Ambion, Inc.). DNA concentration was confirmed using a PicoGreen protocol. RNA quality was confirmed by utilizing an Agilent Bioanalyzer and assigning an RNA Integrity Number (RIN) to each sample.
Label NA
Label protocol Not applicable
Hybridization protocol Total RNA (100 ng) was obtained from 95 of 96 samples, and hybridized to the nCounter Human miRNA sample probes (Seattle, WA).
Scan protocol Each sample was scanned for 600 FOV on the nCounter Digital Analyzer
Description JH1069
Data processing The nanoStringNorm procedure as implemented in the nanoStringNorm Bioconductor package was used to pre-process the miRNA data, followed by differential expression analysis with Empirical Bayes methods via the limma Bioconductor package.
Submission date Jul 27, 2017
Last update date Jul 28, 2017
Contact name leslie cope
Organization name leslie cope
Street address 550 N. Broadway
City Baltimore
State/province MD
ZIP/Postal code 21209
Country USA
Platform ID GPL17904
Series (2)
GSE101976 Molecular analysis of high-grade serous ovarian carcinoma with and without associated serous tubal intra-epithelial carcinoma [miRNA expression]
GSE102094 Molecular analysis of high-grade serous ovarian carcinoma with and without associated serous tubal intra-epithelial carcinoma

Data table header descriptions
VALUE normalized counts

Data table
hsa-let-7a-5p 14.63053063
hsa-let-7b-5p 13.33460836
hsa-let-7c 9.811299817
hsa-let-7d-5p 10.23662579
hsa-let-7e-5p 9.857398301
hsa-let-7f-5p 8.764048854
hsa-let-7g-5p 11.86756252
hsa-let-7i-5p 9.949754386
hsa-miR-1 5.488954673
hsa-miR-100-5p 10.59171976
hsa-miR-101-3p 5.374880241
hsa-miR-103a-3p 5.449507509
hsa-miR-105-5p 4.900894766
hsa-miR-106a-5p+hsa-miR-17-5p 9.491181475
hsa-miR-106b-5p 9.673029874
hsa-miR-107 7.879424607
hsa-miR-10a-5p 7.006838939
hsa-miR-10b-5p 10.92572199
hsa-miR-1178 4.900894766
hsa-miR-1179 3.924479992

Total number of rows: 822

Table truncated, full table size 20 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap