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Sample GSM2719929 Query DataSets for GSM2719929
Status Public on Jul 28, 2017
Title HGSC_JB4757
Sample type RNA
 
Source name primary ovarian tumor tissue
Organism Homo sapiens
Characteristics stic: No
age: 60
path.stage: IIIC
surgical.outcome: Optimal
diagnosis.of.record: Fallopian Tube Ca
diagnosis.after.path.re.review: Fallopian Tube Ca
platinumfreeinterval.mos..: 16.8
platinumstatus: Sensitive
recurrence: Y
pfs.mos..: 21.6
os.mos..: 46.7
vitalstatus: Dead
race: WHITE
tcga_subtype_dif: 0.392
tcga_subtype_imr: 0.372
tcga_subtype_mes: -0.288
tcga_subtype_pro: -0.411
tissue: primary tumor tissue from high grade serous ovarian tumors
Extracted molecule total RNA
Extraction protocol DNA and RNA were co-isolated from frozen tissue tumor blocks, and RNA was isolated from normal tissues utilizing Ambion’s ToTALLY RNA™ RNA Isolation Kit (Part Number AM1910, ©Ambion, Inc.). DNA concentration was confirmed using a PicoGreen protocol. RNA quality was confirmed by utilizing an Agilent Bioanalyzer and assigning an RNA Integrity Number (RIN) to each sample.
Label NA
Label protocol Not applicable
 
Hybridization protocol Total RNA (100 ng) was obtained from 95 of 96 samples, and hybridized to the nCounter Human miRNA sample probes (Seattle, WA).
Scan protocol Each sample was scanned for 600 FOV on the nCounter Digital Analyzer
Description JB4757
Data processing The nanoStringNorm procedure as implemented in the nanoStringNorm Bioconductor package was used to pre-process the miRNA data, followed by differential expression analysis with Empirical Bayes methods via the limma Bioconductor package.
 
Submission date Jul 27, 2017
Last update date Jul 28, 2017
Contact name leslie cope
E-mail(s) cope@jhu.edu
Organization name leslie cope
Street address 550 N. Broadway
City Baltimore
State/province MD
ZIP/Postal code 21209
Country USA
 
Platform ID GPL17904
Series (2)
GSE101976 Molecular analysis of high-grade serous ovarian carcinoma with and without associated serous tubal intra-epithelial carcinoma [miRNA expression]
GSE102094 Molecular analysis of high-grade serous ovarian carcinoma with and without associated serous tubal intra-epithelial carcinoma

Data table header descriptions
ID_REF
VALUE normalized counts

Data table
ID_REF VALUE
hsa-let-7a-5p 13.96620863
hsa-let-7b-5p 11.86756252
hsa-let-7c 8.52893892
hsa-let-7d-5p 8.942404925
hsa-let-7e-5p 9.717372485
hsa-let-7f-5p 9.12549912
hsa-let-7g-5p 12.76832734
hsa-let-7i-5p 9.763157143
hsa-miR-1 5.036542664
hsa-miR-100-5p 9.589347192
hsa-miR-101-3p 4.374087689
hsa-miR-103a-3p 3.898086124
hsa-miR-105-5p 4.543567515
hsa-miR-106a-5p+hsa-miR-17-5p 9.673029874
hsa-miR-106b-5p 8.687225379
hsa-miR-107 7.732089573
hsa-miR-10a-5p 8.614855114
hsa-miR-10b-5p 8.111870904
hsa-miR-1178 5.379910928
hsa-miR-1179 4.80970146

Total number of rows: 822

Table truncated, full table size 20 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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