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Sample GSM2719911 Query DataSets for GSM2719911
Status Public on Jul 28, 2017
Title HGSC_331T
Sample type RNA
Source name primary ovarian tumor tissue
Organism Homo sapiens
Characteristics stic: No
age: 37
path.stage: IIIC
surgical.outcome: Optimal
diagnosis.of.record: Ovarian Ca Ovarian Ca
platinumfreeinterval.mos..: 10.6
platinumstatus: Sensitive
recurrence: Y
pfs.mos..: 16.9
os.mos..: 110.5
vitalstatus: Dead
race: WHITE
tcga_subtype_dif: NA
tcga_subtype_imr: NA
tcga_subtype_mes: NA
tcga_subtype_pro: NA
tissue: primary tumor tissue from high grade serous ovarian tumors
Extracted molecule total RNA
Extraction protocol DNA and RNA were co-isolated from frozen tissue tumor blocks, and RNA was isolated from normal tissues utilizing Ambion’s ToTALLY RNA™ RNA Isolation Kit (Part Number AM1910, ©Ambion, Inc.). DNA concentration was confirmed using a PicoGreen protocol. RNA quality was confirmed by utilizing an Agilent Bioanalyzer and assigning an RNA Integrity Number (RIN) to each sample.
Label NA
Label protocol Not applicable
Hybridization protocol Total RNA (100 ng) was obtained from 95 of 96 samples, and hybridized to the nCounter Human miRNA sample probes (Seattle, WA).
Scan protocol Each sample was scanned for 600 FOV on the nCounter Digital Analyzer
Description 331T
Data processing The nanoStringNorm procedure as implemented in the nanoStringNorm Bioconductor package was used to pre-process the miRNA data, followed by differential expression analysis with Empirical Bayes methods via the limma Bioconductor package.
Submission date Jul 27, 2017
Last update date Jul 28, 2017
Contact name leslie cope
Organization name leslie cope
Street address 550 N. Broadway
City Baltimore
State/province MD
ZIP/Postal code 21209
Country USA
Platform ID GPL17904
Series (2)
GSE101976 Molecular analysis of high-grade serous ovarian carcinoma with and without associated serous tubal intra-epithelial carcinoma [miRNA expression]
GSE102094 Molecular analysis of high-grade serous ovarian carcinoma with and without associated serous tubal intra-epithelial carcinoma

Data table header descriptions
VALUE normalized counts

Data table
hsa-let-7a-5p 12.59047758
hsa-let-7b-5p 11.64692345
hsa-let-7c 7.91053449
hsa-let-7d-5p 10.35921118
hsa-let-7e-5p 9.12549912
hsa-let-7f-5p 7.547849985
hsa-let-7g-5p 11.35498183
hsa-let-7i-5p 7.91053449
hsa-miR-1 5.921884773
hsa-miR-100-5p 8.414125739
hsa-miR-101-3p 4.548306822
hsa-miR-103a-3p 4.238191512
hsa-miR-105-5p 5.68232786
hsa-miR-106a-5p+hsa-miR-17-5p 9.327466015
hsa-miR-106b-5p 9.237368735
hsa-miR-107 5.921884773
hsa-miR-10a-5p 9.589347192
hsa-miR-10b-5p 10.05953709
hsa-miR-1178 6.048353217
hsa-miR-1179 5.471461307

Total number of rows: 822

Table truncated, full table size 20 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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