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Sample GSM270189 Query DataSets for GSM270189
Status Public on May 12, 2008
Title CE9
Sample type SRA
 
Source name 9 day embryo
Organism Gallus gallus
Characteristics breed: Ross-308™, Tissue:whole embryo, Stage:9 days of incubation
Growth protocol Fertilized Ross-308 eggs were incubated in a Multiquip incubator at 37.5 C with rotation every 6 hours. Chick embryos were collected at day 5, 7, and 9 of incubation to represent the embryonic developmental stages 25-27, 30-31, and 35 respectively (Hamburger and Hamilton 1992). After removal of the amnion embryos were rinsed in 1xPBS, and immediately processed for RNA isolation.
Extracted molecule total RNA
Extraction protocol Low molecular weight RNA was extracted using mirVana Isolation Kit (Ambion) folowing the manufacturer's protocol.
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina Genome Analyzer
 
Description 9 day embryo
Data processing Refer to Supplemental Methods of the related manuscript for detail.
 
Submission date Mar 02, 2008
Last update date May 15, 2019
Contact name Evgeny Glazov
E-mail(s) e.glazov@uq.edu.au
Organization name University of Queensland
Department Diamantina Institute
Street address Ipswich Road
City Brisbane
ZIP/Postal code 4102
Country Australia
 
Platform ID GPL10223
Series (1)
GSE10686 Deep sequencing of small RNA libraries from chicken embryo
Relations
SRA SRX029065
BioSample SAMN00116358

Data table header descriptions
SEQUENCE
COUNT count

Data table
SEQUENCE COUNT
TGAGGTAGTAGGTTGTATAGTTTCGTATGCCGT 384951
TGAGGTAGTAGATTGTATAGTTTCGTATGCCGT 287397
TGGAATGTAAAGAAGTATGTATTCGTATGCCGT 234481
TGAGGTAGTAGGTTGTATGGTTTCGTATGCCGT 174506
TGGAGTGTGACAATGGTGTTTGTCGTATGCCGT 112922
ACAGTAGTCTGCACATTGGTTATCGTATGCCGT 79456
ACAGTAGTCTGCACATTGGTTTCGTATGCCGTC 61388
TGGAATGTAAGGAAGTGTGTGGTCGTATGCCGT 60933
TAAGGCACGCGGTGAATGCCAATCGTATGCCGT 47777
TGAGGTAGTAGATTGTATAGTTTCGTATTCCGT 47296
TGAGGTAGTAGGTTGTATAGTTTCGTATTCCGT 46364
AGCAGCATTGTACAGGGCTATGATCGTATGCCG 32694
TGAGGTAGTAGTTTGTACAGTTTCGTATGCCGT 32449
AGCAGCATTGTACAGGGCTATTCGTATGCCGTC 28023
TGAGGTAGTAGGTTGTGTGGTTTCGTATGCCGT 26394
ACCACAGGGTAGAACCACGGATCGTATGCCGTC 25049
TCTTTGGTTATCTAGCTGTATGATCGTATGCCG 23581
TCACAGTGAACCGGTCTCTTTTCGTATGCCGTC 23301
TACCACAGGGTAGAACCACGGACTCGTATGCCG 23002
CAGGCTGGTTAGATGGTTGTCTTCGTATGCCGT 22941

Total number of rows: 153406

Table truncated, full table size 5400 Kbytes.




Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data included within Sample table
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