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Sample GSM270188 Query DataSets for GSM270188
Status Public on May 12, 2008
Title CE7
Sample type SRA
 
Source name 7 day embryo
Organism Gallus gallus
Characteristics breed: Ross-308™, Tissue:whole embryo, Stage:7 days of incubation
Growth protocol Fertilized Ross-308 eggs were incubated in a Multiquip incubator at 37.5 C with rotation every 6 hours. Chick embryos were collected at day 5, 7, and 9 of incubation to represent the embryonic developmental stages 25-27, 30-31, and 35 respectively (Hamburger and Hamilton 1992). After removal of the amnion embryos were rinsed in 1xPBS, and immediately processed for RNA isolation.
Extracted molecule total RNA
Extraction protocol Low molecular weight RNA was extracted using mirVana Isolation Kit (Ambion) folowing the manufacturer's protocol.
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina Genome Analyzer
 
Description 7 day embryo
Data processing Refer to Supplemental Methods of the related manuscript for detail.
 
Submission date Mar 02, 2008
Last update date May 15, 2019
Contact name Evgeny Glazov
E-mail(s) e.glazov@uq.edu.au
Organization name University of Queensland
Department Diamantina Institute
Street address Ipswich Road
City Brisbane
ZIP/Postal code 4102
Country Australia
 
Platform ID GPL10223
Series (1)
GSE10686 Deep sequencing of small RNA libraries from chicken embryo
Relations
SRA SRX029064
BioSample SAMN00116357

Data table header descriptions
SEQUENCE
COUNT count

Data table
SEQUENCE COUNT
TGAGGTAGTAGGTTGTATAGTTTCGTATGCCGT 340155
TGAGGTAGTAGATTGTATAGTTTCGTATGCCGT 226956
TGGAATGTAAAGAAGTATGTATTCGTATGCCGT 204090
TGAGGTAGTAGGTTGTATGGTTTCGTATGCCGT 144788
ACAGTAGTCTGCACATTGGTTTCGTATGCCGTC 98309
ACAGTAGTCTGCACATTGGTTATCGTATGCCGT 90223
TGGAGTGTGACAATGGTGTTTGTCGTATGCCGT 88623
TGGAATGTAAGGAAGTGTGTGGTCGTATGCCGT 54912
TCACAGTGAACCGGTCTCTTTTCGTATGCCGTC 44438
AGCAGCATTGTACAGGGCTATGATCGTATGCCG 40164
ACCACAGGGTAGAACCACGGATCGTATGCCGTC 39078
ACCACAGGGTAGAACCACGGACTCGTATGCCGT 33168
AGCAGCATTGTACAGGGCTATTCGTATGCCGTC 31684
TGAGGTAGTAGTTTGTACAGTTTCGTATGCCGT 30462
AGCAGCATTGTACAGGGCTATCATCGTATGCCG 26590
GAGGAAGAAGGAATATTTTTCGTATGCCGTCTT 26181
TGAGGTAGTAGGTTGTATAGTTTCGTATTCCGT 25377
TAAGGCACGCGGTGAATGCCAATCGTATGCCGT 24609
TGAGGTAGTAGATTGTATAGTTTCGTATTCCGT 23373
CAGGCTGGTTAGATGGTTGTCTTCGTATGCCGT 22199

Total number of rows: 220166

Table truncated, full table size 7749 Kbytes.




Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data included within Sample table
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