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Sample GSM2700108 Query DataSets for GSM2700108
Status Public on Jul 12, 2017
Title total RNA-seq from G1E (ENCLB924EVD)
Sample type SRA
 
Source name Mus musculus strain C57BL/6J G1E immortalized cell line
Organism Mus musculus
Characteristics line: G1E
biomaterial_type: immortalized cell line
description: Gata1- erythroid progenitor
biosample encode accession: ENCBS159ZTO (SAMN07278450)
dev stage: postnatal
lab: Ross Hardison, PennState
age: 0 day
Sex: male
link: ENCBS159ZTO at ENCODE; https://www.encodeproject.org/ENCBS159ZTO/
link: Derived from ENCODE donor ENCDO072AAA; https://www.encodeproject.org/ENCDO072AAA/
link: general protocol; https://www.encodeproject.org/documents/a44c24de-0197-480f-baa4-3ec963213578/@@download/attachment/G1E_Hardison_protocol.pdf
Extracted molecule total RNA
Extraction protocol extraction protocol: https://www.encodeproject.org/documents/0ab38aa8-888b-4ab3-aecf-13a096af7fd7/@@download/attachment/purelink_rna_mini_kit_man.pdf, general protocol: https://www.encodeproject.org/documents/17c31e10-1542-42c6-8b4c-3afff95564cf/@@download/attachment/scriptseq-complete-kit-human-mouse-rat-low-input-library-prep-guide.pdf, general protocol: https://www.encodeproject.org/documents/a44c24de-0197-480f-baa4-3ec963213578/@@download/attachment/G1E_Hardison_protocol.pdf
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description https://www.encodeproject.org/experiments/ENCSR475TDY/
***************
biological replicate number: 1
technical replicate number: 1
description: PSU mouse G1E 100ng rRNA-depeleted RNA-seq via ScriptSeq
experiment encode accession: ENCSR475TDY
assay title: total RNA-seq
assembly: mm10
encode release date: 2017-01-04
lab: Ross Hardison, PennState
library encode accession: ENCLB924EVD
size range: 200-1000
nucleic acid starting quantity: 100
nucleic acid starting quantity units: ng
Data processing See GSM*_README.txt supplementary file linked below
 
Submission date Jul 11, 2017
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL19057
Series (1)
GSE101173 total RNA-seq from G1E (ENCSR475TDY)
Relations
BioSample SAMN07278450
SRA SRX3010044
Named Annotation GSM2700108_ENCFF250DBI_plus_strand_signal_of_unique_reads_mm10.bigWig
Named Annotation GSM2700108_ENCFF118JHV_minus_strand_signal_of_all_reads_mm10.bigWig
Named Annotation GSM2700108_ENCFF932JCD_minus_strand_signal_of_unique_reads_mm10.bigWig
Named Annotation GSM2700108_ENCFF830TOQ_plus_strand_signal_of_all_reads_mm10.bigWig

Supplementary file Size Download File type/resource
GSM2700108_ENCFF118JHV_minus_strand_signal_of_all_reads_mm10.bigWig 261.9 Mb (ftp)(http) BIGWIG
GSM2700108_ENCFF250DBI_plus_strand_signal_of_unique_reads_mm10.bigWig 216.4 Mb (ftp)(http) BIGWIG
GSM2700108_ENCFF415TIY_transcript_quantifications_mm10.tsv.gz 4.4 Mb (ftp)(http) TSV
GSM2700108_ENCFF830TOQ_plus_strand_signal_of_all_reads_mm10.bigWig 259.2 Mb (ftp)(http) BIGWIG
GSM2700108_ENCFF932JCD_minus_strand_signal_of_unique_reads_mm10.bigWig 221.9 Mb (ftp)(http) BIGWIG
GSM2700108_ENCFF997XIM_gene_quantifications_mm10.tsv.gz 2.0 Mb (ftp)(http) TSV
GSM2700108_ENCLB924EVD_README.txt.gz 751 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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