|
Status |
Public on Feb 20, 2009 |
Title |
UIP, biological rep13 |
Sample type |
RNA |
|
|
Source name |
Lung tissue with usual interstitial pneumonia (UIP), biological rep13
|
Organism |
Homo sapiens |
Characteristics |
Tissue from a patient with pathological diagnosis of usual interstitial pneumonia.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA followed by RNeasy cleanup was performed according to the manufacturers' instructions.
|
Label |
Cy3
|
Label protocol |
Cy3 labeled cRNA were prepared according to the standard Agilent protocol from 500ng total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, 1.5 ug of cRNA were hybridized for 17 hr at 65C on Agilent 4x44k Whole Human Genome Array. Arrays were washed according to the manufacturer's protocol.
|
Scan protocol |
Agilent Scanner protocol
|
Description |
Lung tissue samples obtained from the University of Pittsburgh Health Sciences Tissue Bank. Gene expression data from a lung with usual interstitial pneumonia.
|
Data processing |
Agilent Feature Extraction
|
|
|
Submission date |
Feb 28, 2008 |
Last update date |
Mar 26, 2008 |
Contact name |
Kazuhisa Konishi |
E-mail(s) |
konishi.kazuhisa@med.osaka-cu.ac.jp
|
Phone |
412-647-3156
|
Organization name |
University of Pittsburgh
|
Department |
Pulmonry, Allergy, and Critical Care Medicine
|
Lab |
Kaminski Lab
|
Street address |
1212 Kaufmann Building 3471 Fifth Ave
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15213 |
Country |
USA |
|
|
Platform ID |
GPL4133 |
Series (1) |
GSE10667 |
MMP1 and MMP7 as potential peripheral blood biomarkers in Idiopathic Pulmonary Fibrosis |
|