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Sample GSM2695308 Query DataSets for GSM2695308
Status Public on Aug 10, 2017
Title IL22KO_asthma
Sample type SRA
 
Source name Lung
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: Lung
genotype: IL22-/-
Treatment protocol [WT_asthma and IL22KO_asthma] WT and IL22KO mice were sensitized and challenged with HDM. Forty-eight hours after the last HDM administration, total RNAs were prepared from the lung. [PBS_Lung and IL22_Lung] PBS and recombinant IL-22 was intratracheally administered to WT mice. Twenty-four hours after the administration, total RNAs were prepared from the lung.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen lungs using PureLink RNA Mini Kit (invitrogen)
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Description asthma_processed.txt
Data processing CASAVA 1.8.2 software used for base-calling
Default parameters of Tophat2 use for alignment
Cufflinks 2.2.1 used for FPKM normalization
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include FPKM values
 
Submission date Jul 06, 2017
Last update date May 15, 2019
Contact name Takashi Ito
E-mail(s) sueitoh@chiba-u.jp
Organization name Chiba university
Street address Inohana
City Chiba
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL18480
Series (1)
GSE100858 IL-22 inhibits allergic airway inflammation in house dust mite-induced asthma models
Relations
BioSample SAMN07327425
SRA SRX2987120

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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